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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LGALS13 Polyclonal antibody (17823-1-AP)

    WB analysis using 17823-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17823-1-AP (LGALS13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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LGALS13 Polyclonal antibody

LGALS13 Polyclonal Antibody for WB, ELISA
Cat No. 17823-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Galectin-13, Galectin 13, Galactoside-binding soluble lectin 13, Gal-13, GAL13

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17823-1-AP targets LGALS13 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LGALS13 fusion protein Ag12192 相同性解析による交差性が予測される生物種
Full Name lectin, galactoside-binding, soluble, 13
Calculated molecular weight 139 aa, 16 kDa
Observed molecular weight16 kDa
GenBank accession numberBC066304
Gene Symbol LGALS13
Gene ID (NCBI) 29124
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHV8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Galectins are glycan-binding proteins that regulate innate and adaptive immune responses, and some confer maternal-fetal immune tolerance in eutherian mammals.  Three of the five human cluster galectins are solely expressed in the placenta, where they may confer additional immunoregulatory functions to enable deep placentation (PMID: 25191322). One of these is galactoside-binding soluble lectin 13 (LGALS13, also known as Galectin-13 and PP13), which has a high affinity for the sugar residues of glycoproteins. PP13 is implicated in placentation and is involved in inflammation and immune defense required for trophoblast invasion during uterine artery remodeling in early pregnancy (PMID: 19497882; 10527825; 21596368; 26018511).

Protocols

Product Specific Protocols
WB protocol for LGALS13 antibody 17823-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols