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LGALS3BP Polyclonal antibody

Name: LGALS3BP Polyclonal antibody
Brand: Proteintech
SKU: 10281-1-AP
Rating: 5.00 (1 reviews)

LGALS3BP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 10281-1-AP

Synonyms

M2BP, Lectin galactoside-binding soluble 3-binding protein, Galectin-3-binding protein, Galectin 3 binding protein, BTBD17B

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 10281-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10281-1-AP
WB result of LGALS3BP antibody (10281-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LGALS3BP transfected HepG2 cells.
WB analysis of fetal human brain using 10281-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10281-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 10281-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10281-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 10281-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10281-1-AP (LGALS3BP Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 10281-1-AP
IP result of anti-LGALS3BP (IP:10281-1-AP, 4ug; Detection:10281-1-AP 1:1000) with HepG2 cells lysate 1085 ug.
IP experiment of HEK-293 using 10281-1-AP
IP result of anti-LGALS3BP (IP:10281-1-AP, 4ug; Detection:10281-1-AP 1:400) with HEK-293 cells lysate 1000ug.
IHC staining of human oesophagus cancer using 10281-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10281-1-AP (LGALS3BP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human oesophagus cancer using 10281-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10281-1-AP (LGALS3BP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10281-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 10281-1-AP (LGALS3BP antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10281-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10281-1-AP (LGALS3BP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10281-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10281-1-AP (LGALS3BP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10281-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10281-1-AP (LGALS3BP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 10281-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LGALS3BP antibody (10281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 10281-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, COLO 320 cells, HEK-293 cells, fetal human brain tissue, HepG2 cells, A549 cells, human milk tissue, human plasma tissue
Positive IP detected in	HepG2 cells, HEK-293 cells
Positive IHC detected in	human oesophagus cancer tissue, human breast cancer tissue, human colon cancer, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 10 publications below
IHC	See 6 publications below
IF	See 4 publications below
CoIP	See 1 publications below

Product Information

10281-1-AP targets LGALS3BP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LGALS3BP fusion protein Ag0294 相同性解析による交差性が予測される生物種
Full Name	lectin, galactoside-binding, soluble, 3 binding protein
Calculated molecular weight	585 aa, 65 kDa
Observed molecular weight	65-90 kDa
GenBank accession number	BC002403
Gene symbol	LGALS3BP
Gene ID (NCBI)	3959
RRID	AB_2137066
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ).

Protocols

Product Specific Protocols
WB protocol for LGALS3BP antibody 10281-1-AP	Download protocol
IHC protocol for LGALS3BP antibody 10281-1-AP	Download protocol
IF protocol for LGALS3BP antibody 10281-1-AP	Download protocol
IP protocol for LGALS3BP antibody 10281-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2. Authors - Chunqing Wang View Article
human	IHC	Cancer Res A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma. Authors - Silverman Ayaka M AM KD Validated View Article
human	IF	Blood Adv Increased Mac-2 binding protein glycan isomer in patients at risk for late nonrelapse mortality after HSCT. Authors - Yu Akahoshi View Article
mouse	WB	PLoS Pathog Inducible LGALS3BP/90K activates antiviral innate immune responses by targeting TRAF6 and TRAF3 complex. Authors - Gang Xu View Article
human	WB	Thromb Haemost Proteomic discovery of 21 proteins expressed in human plasma-derived but not platelet-derived microparticles. Authors - Smalley David M DM View Article
human	IHC	Cancer Sci MZT2A promotes NSCLC viability and invasion by increasing Akt phosphorylation via the MOZART2 domain. Authors - Huanxi Wang View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Emma (Verified Customer) (03-15-2022)

Beautiful antibody - produces a single band at the correct size.

Applications: Western Blot
Primary Antibody Dilution: 1:1000
Cell Tissue Type: LNCaP

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LGALS3BP Polyclonal antibody

LGALS3BP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 10281-1-AP

WB analysis of HepG2 using 10281-1-AP

WB analysis of fetal human brain using 10281-1-AP

WB analysis of HeLa using 10281-1-AP

WB analysis of COLO 320 using 10281-1-AP

WB analysis of HEK-293 using 10281-1-AP

WB analysis of A549 using 10281-1-AP

IP experiment of HepG2 using 10281-1-AP

IP experiment of HEK-293 using 10281-1-AP

IHC staining of human oesophagus cancer using 10281-1-AP

IHC staining of human oesophagus cancer using 10281-1-AP

IHC staining of human colon cancer using 10281-1-AP

IHC staining of human colon cancer using 10281-1-AP

IHC staining of human breast cancer using 10281-1-AP

IHC staining of human breast cancer using 10281-1-AP

IF Staining of HepG2 using 10281-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma.

Increased Mac-2 binding protein glycan isomer in patients at risk for late nonrelapse mortality after HSCT.

Inducible LGALS3BP/90K activates antiviral innate immune responses by targeting TRAF6 and TRAF3 complex.

Proteomic discovery of 21 proteins expressed in human plasma-derived but not platelet-derived microparticles.

MZT2A promotes NSCLC viability and invasion by increasing Akt phosphorylation via the MOZART2 domain.

Reviews

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