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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Galectin-4 Polyclonal antibody

Galectin-4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13391-1-AP

Synonyms

Galectin 4, Gal-4, GAL4, Gal 4, Antigen NY-CO-27

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Galectin-4 Polyclonal antibody (13391-1-AP)

    WB analysis using 13391-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 13391-1-AP (Galectin-4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using Galectin-4 Polyclonal antibody (13391-1-AP)

    WB analysis of COLO 320 using 13391-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13391-1-AP (Galectin-4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Galectin-4 Polyclonal antibody (13391-1-AP)

    IHC staining of human breast cancer using 13391-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13391-1-AP (Galectin-4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Galectin-4 Polyclonal antibody (13391-1-AP)

    IHC staining of human breast cancer using 13391-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13391-1-AP (Galectin-4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of COLO 320 cells using Galectin-4 Polyclonal antibody (13391-1-AP)

    IF Staining of COLO 320 using 13391-1-AP

    Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using Galectin-4 antibody (13391-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHT-29 cells, COLO 320 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13391-1-AP targets Galectin-4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Galectin-4 fusion protein Ag4049 相同性解析による交差性が予測される生物種
Full Name lectin, galactoside-binding, soluble, 4
Calculated molecular weight 323 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC034750
Gene symbol Galectin-4
Gene ID (NCBI) 3960
RRIDAB_2137072
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-4 contains two arbohydrate recognition domains (CRDs) and is expressed in the epithelium of oral mucosa, esophagus, and intestinal and colonic mucosa (PMID: 15546874; 15115909). Aberrant expression of galectin-4 has been reported in several cancers.

Protocols

Product Specific Protocols
WB protocol for Galectin-4 antibody 13391-1-APDownload protocol
IHC protocol for Galectin-4 antibody 13391-1-APDownload protocol
IF protocol for Galectin-4 antibody 13391-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols