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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Galectin 8 Polyclonal antibody

Galectin 8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10955-1-AP

Synonyms

LGALS8, Po66 carbohydrate-binding protein, PCTA-1, Galectin-8, Gal-8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Galectin 8 Polyclonal antibody (10955-1-AP)

    WB analysis using 10955-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10955-1-AP (Galectin 8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using Galectin 8 Polyclonal antibody (10955-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 10955-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10955-1-AP (Galectin 8 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Galectin 8 Polyclonal antibody (10955-1-AP)

    IF Staining of HEK-293 using 10955-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Galectin 8 antibody (10955-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10955-1-AP targets Galectin 8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Galectin 8 fusion protein Ag1387 相同性解析による交差性が予測される生物種
Full Name lectin, galactoside-binding, soluble, 8
Calculated molecular weight 34 kDa
Observed molecular weight36 kDa, 40 kDa
GenBank accession numberBC015818
Gene symbol Galectin 8
Gene ID (NCBI) 3964
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Galectin 8 is a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions.

Protocols

Product Specific Protocols
WB protocol for Galectin 8 antibody 10955-1-APDownload protocol
IHC protocol for Galectin 8 antibody 10955-1-APDownload protocol
IF protocol for Galectin 8 antibody 10955-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols