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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using LGI1 Polyclonal antibody (12483-1-AP)

    WB analysis of human brain using 12483-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12483-1-AP (LGI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LGI1 Polyclonal antibody (12483-1-AP)

    WB analysis of HeLa using 12483-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12483-1-AP (LGI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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LGI1 Polyclonal antibody

LGI1 Polyclonal Antibody for WB, ELISA
Cat No. 12483-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EPITEMPIN, Epitempin 1, EPT, ETL1, IB1099, LGI1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman brain tissue, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12483-1-AP targets LGI1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LGI1 fusion protein Ag3184 相同性解析による交差性が予測される生物種
Full Name leucine-rich, glioma inactivated 1
Calculated molecular weight 557 aa, 64 kDa
Observed molecular weight 64 kDa, 58 kDa
GenBank accession numberBC022500
Gene Symbol LGI1
Gene ID (NCBI) 9211
RRIDAB_10637849
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95970
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LGI1 antibody 12483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols