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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LGMN Polyclonal antibody (15095-1-AP)

    WB analysis using 15095-1-AP (same clone as 15095-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15095-1-AP (LGMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15095-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using LGMN Polyclonal antibody (15095-1-AP)

    IHC staining of mouse kidney using 15095-1-AP (same clone as 15095-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15095-1-AP (LGMN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15095-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using LGMN Polyclonal antibody (15095-1-AP)

    IHC staining of mouse kidney using 15095-1-AP (same clone as 15095-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15095-1-AP (LGMN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15095-1-PBS in a different storage buffer formulation.

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LGMN Polyclonal antibody, PBS Only

LGMN Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15095-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15095-1-PBS targets LGMN in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LGMN fusion protein Ag7170 相同性解析による交差性が予測される生物種
Full Name legumain
Calculated molecular weight 49 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC003061
Gene Symbol LGMN
Gene ID (NCBI) 5641
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99538
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

LGMN (Legumain), also known as asparaginyl endopeptidase or AEP, is a broadly expressed lysosomal cysteine endopeptidase that belongs to peptidase family C13 and specifcally hydrolyzes substrate asparaginyl bonds (PMID: 9065484). LGMN directly regulates diverse physiological and pathological processes by remodeling tissue-specific targets (PMID: 25205715, 21292981, 18820679). In addition, LGMN indirectly contributes to atherosclerotic plaque instability through activation of cathepsin L in the arterial ECM (PMID: 19671471, 18377911). LGMN is secreted as inactive prolegumain (56 kDa) and processed into enzymatically active 46 and 36 kDa forms, as well as a 17 kDa enzymatically inactive C-terminal fragment (PMID: 28162997, PMID: 19671471).