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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LGMN Monoclonal antibody, PBS Only

LGMN Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2B12

Cat no : 67017-1-PBS

Synonyms

AEP, Asparaginyl endopeptidase, legumain, LGMN, LGMN1, Protease, cysteine 1, PRSC1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LGMN Monoclonal antibody (67017-1-Ig)

    WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LGMN Monoclonal antibody (67017-1-Ig)

    WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LGMN Monoclonal antibody (67017-1-Ig)

    WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using LGMN Monoclonal antibody (67017-1-Ig)

    WB analysis of MCF-7 using 67017-1-Ig (same clone as 67017-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig kidney tissue using LGMN Monoclonal antibody (67017-1-Ig)

    WB analysis of pig kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using LGMN Monoclonal antibody (67017-1-Ig)

    WB analysis of human placenta using 67017-1-Ig (same clone as 67017-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using LGMN Monoclonal antibody (67017-1-Ig)

    WB analysis of HEK-293 using 67017-1-Ig (same clone as 67017-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using LGMN Monoclonal antibody (67017-1-Ig)

    WB analysis of NCCIT using 67017-1-Ig (same clone as 67017-1-PBS)

    NCCIT cell were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig kidney tissue using LGMN Monoclonal antibody (67017-1-Ig)

    WB analysis of pig kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using LGMN Monoclonal antibody (67017-1-Ig)

    IHC staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using LGMN Monoclonal antibody (67017-1-Ig)

    IHC staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using LGMN Monoclonal antibody (67017-1-Ig)

    IHC staining of human placenta using 67017-1-Ig (same clone as 67017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using LGMN Monoclonal antibody (67017-1-Ig)

    IHC staining of human placenta using 67017-1-Ig (same clone as 67017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using LGMN Monoclonal antibody (67017-1-Ig)

    IF Staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using LGMN antibody (67017-1-Ig, Clone: 1F2B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using LGMN Monoclonal antibody (67017-1-Ig)

    IF Staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using LGMN antibody (67017-1-Ig, Clone: 1F2B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67017-1-PBS targets LGMN in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, pig, mouse samples.

Tested Reactivity Human, pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LGMN fusion protein Ag7218 相同性解析による交差性が予測される生物種
Full Name legumain
Calculated molecular weight 49 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC003061
Gene symbol LGMN
Gene ID (NCBI) 5641
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LGMN (Legumain), also known as asparaginyl endopeptidase or AEP, is a broadly expressed lysosomal cysteine endopeptidase that belongs to peptidase family C13 and specifcally hydrolyzes substrate asparaginyl bonds (PMID: 9065484). LGMN directly regulates diverse physiological and pathological processes by remodeling tissue-specific targets (PMID: 25205715, 21292981, 18820679). In addition, LGMN indirectly contributes to atherosclerotic plaque instability through activation of cathepsin L in the arterial ECM (PMID: 19671471, 18377911). LGMN is secreted as inactive prolegumain (56 kDa) and processed into enzymatically active 46 and 36 kDa forms, as well as a 17 kDa enzymatically inactive C-terminal fragment (PMID: 28162997, PMID: 19671471).