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Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using LGR6 Polyclonal antibody (17658-1-AP)

    WB analysis of HUVEC using 17658-1-AP

    HUVEC cells were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using LGR6 Polyclonal antibody (17658-1-AP)

    WB analysis of mouse testis using 17658-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LGR6 Polyclonal antibody (17658-1-AP)

    WB analysis using 17658-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using LGR6 Polyclonal antibody (17658-1-AP)

    WB analysis of human brain using 17658-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17658-1-AP (LGR6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using LGR6 Polyclonal antibody (17658-1-AP)

    IHC staining of human testis using 17658-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17658-1-AP (LGR6 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using LGR6 Polyclonal antibody (17658-1-AP)

    IHC staining of human testis using 17658-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17658-1-AP (LGR6 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using LGR6 Polyclonal antibody (17658-1-AP)

    IHC staining of human ovary using 17658-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using LGR6 Polyclonal antibody (17658-1-AP)

    IHC staining of human ovary using 17658-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using LGR6 Polyclonal antibody (17658-1-AP)

    IHC staining of human spleen using 17658-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using LGR6 Polyclonal antibody (17658-1-AP)

    IHC staining of human spleen using 17658-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17658-1-AP (LGR6 antibody) at dilution of 1:50 (under 40x lens).

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  • Featured Product
  • KD/KO Validated

LGR6 Polyclonal antibody

LGR6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17658-1-AP
Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

VTS20631, UNQ6427/PRO21331, Leucine-rich repeat-containing G-protein coupled receptor 6, GPCR

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHUVEC cells, human brain tissue, mouse testis tissue, HeLa cells, LNCaP cells, PC-3 cells
Positive IHC detected inhuman testis tissue, human spleen tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17658-1-AP targets LGR6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LGR6 fusion protein Ag11909 相同性解析による交差性が予測される生物種
Full Name leucine-rich repeat-containing G protein-coupled receptor 6
Calculated molecular weight 915 aa, 99 kDa
Observed molecular weight 104 kDa
GenBank accession numberBC047905
Gene Symbol LGR6
Gene ID (NCBI) 59352
RRIDAB_2135163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBX8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LGR6 (Leucine-rich repeat-containing G-protein coupled receptor 6) is a seven-transmembrane protein. LGR6 is a high affinity receptor of R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. It potentially functions as a tumor suppressor. (PMID: 20223988; 20417836; 22615920)

Protocols

Product Specific Protocols
WB protocol for LGR6 antibody 17658-1-APDownload protocol
IHC protocol for LGR6 antibody 17658-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

J Clin Invest

Maresin 1 activates LGR6 receptor promoting phagocyte immunoresolvent functions.

Authors - Nan Chiang
  • KD Validated
View Article
humanWB

Int J Biol Macromol

Study on the mechanism of macrophages activated by phosphoesterified rehmanniae polysaccharide on human gastric cancer cells

Authors - Xianglong Liu
View Article
humanWB

Mol Ther Oncolytics

Silencing LGR6 Attenuates Stemness and Chemoresistance via Inhibiting Wnt/β-Catenin Signaling in Ovarian Cancer.

Authors - Xiaohong Ruan
View Article
humanWB

PLoS One

Characterizing the cellular attachment receptor for Langat virus.

Authors - Raquel Rodrigues
View Article
mouseWB

JBMR Plus

Evidence for Lgr6 as a Novel Marker of Osteoblastic Progenitors in Mice.

Authors - Vikram Khedgikar
View Article
mouseELISA

Proc Natl Acad Sci U S A

The Maresin 1-LGR6 axis decreases respiratory syncytial virus-induced lung inflammation

Authors - Nandini Krishnamoorthy
View Article