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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LHPP Monoclonal antibody

LHPP Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Mouse, Rat, Pig, human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A2A4

Cat no : 66988-1-Ig

Synonyms

hLHPP, LHPP

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of rat liver tissue using LHPP Monoclonal antibody (66988-1-Ig)

    WB analysis of rat liver using 66988-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66988-1-Ig (LHPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LHPP Monoclonal antibody (66988-1-Ig)

    WB analysis of mouse brain using 66988-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66988-1-Ig (LHPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using LHPP Monoclonal antibody (66988-1-Ig)

    WB analysis of pig liver using 66988-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66988-1-Ig (LHPP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LHPP Monoclonal antibody (66988-1-Ig)

    IF Staining of HepG2 using 66988-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LHPP antibody (66988-1-Ig, Clone: 2A2A4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inrat liver tissue, pig liver tissue, mouse brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66988-1-Ig targets LHPP in WB, IF/ICC, ELISA applications and shows reactivity with Mouse, Rat, Pig, human samples.

Tested Reactivity Mouse, Rat, Pig, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LHPP fusion protein Ag8375 相同性解析による交差性が予測される生物種
Full Name phospholysine phosphohistidine inorganic pyrophosphate phosphatase
Calculated molecular weight 23 kDa, 29 kDa
Observed molecular weight 23-30 kDa
GenBank accession numberBC007324
Gene symbol LHPP
Gene ID (NCBI) 64077
RRIDAB_2882305
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LHPP antibody 66988-1-IgDownload protocol
IF protocol for LHPP antibody 66988-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols