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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using LHX2 Polyclonal antibody (18133-1-AP)

    WB analysis of Jurkat using 18133-1-AP

    WB result of LHX2 antibody (18133-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LHX2 transfected Jurkat cells.

  • Western Blot (WB) analysis of various lysates using LHX2 Polyclonal antibody (18133-1-AP)

    WB analysis using 18133-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18133-1-AP (LHX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  • KD/KO Validated

LHX2 Polyclonal antibody

LHX2 Polyclonal Antibody for WB, ELISA
Cat No. 18133-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

hLhx2, Homeobox protein LH 2, LH2, LHX2, LIM homeobox 2, LIM homeobox protein 2, LIM/homeobox protein Lhx2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, Jurkat cells, Daudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18133-1-AP targets LHX2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LHX2 fusion protein Ag12717 相同性解析による交差性が予測される生物種
Full Name LIM homeobox 2
Calculated molecular weight 406 aa, 44 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC093662
Gene Symbol LHX2
Gene ID (NCBI) 9355
RRIDAB_3085555
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50458
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LHX proteins can activate or repress gene transcription by direct DNA binding and association with co-activators or co-repressors through their LIM domain. The LIM homeobox 2 (LHX2), also known as LH2, is a member of the LIM homeodomain family of proteins. LHX2 functions as a nuclear transcription co-factor and is reported to be involved in many important physiological processes, such as cell division, cell proliferation, specific cell-type differentiation, and cerebral cortex development. LHX2 promotes the growth and metastasis of nasopharyngeal carcinoma by regulating Wnt signaling and plays a vital role in breast cancer's progression and prognosis(PMID: 37345110).

Protocols

Product Specific Protocols
WB protocol for LHX2 antibody 18133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols