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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LIAS Monoclonal antibody, PBS Only

LIAS Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3B5G7

Cat no : 67298-1-PBS

Synonyms

Lip syn, LAS, HUSSY-01, HUSSY 01, EC:2.8.1.8

Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using LIAS Monoclonal antibody (67298-1-Ig)

    WB analysis of LNCaP using 67298-1-Ig (same clone as 67298-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67298-1-Ig (LIAS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67298-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LIAS Monoclonal antibody (67298-1-Ig)

    WB analysis using 67298-1-Ig (same clone as 67298-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67298-1-Ig (LIAS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67298-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LIAS Monoclonal antibody (67298-1-Ig)

    IHC staining of mouse brain using 67298-1-Ig (same clone as 67298-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67298-1-Ig (LIAS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67298-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67298-1-PBS targets LIAS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LIAS fusion protein Ag27286 相同性解析による交差性が予測される生物種
Full Name lipoic acid synthetase
Calculated molecular weight 372 aa, 42 kDa
Observed molecular weight 34-42 kDa
GenBank accession numberBC023635
Gene symbol LIAS
Gene ID (NCBI) 11019
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LIAS(lipoyl synthase, mitochondrial) is also named as LAS and belongs to the radical SAM superfamily and lipoyl synthase family. It produces alpha-lipoic acid, an antioxidant and an essential cofactor in alpha-ketoacid dehydrogenase complexes, which participate in glucose oxidation and ATP generation(PMID:22021711). The deduced 373-amino acid protein has a calculated molecular mass of about 42 kD. The N-terminal 26 amino acids encode a potential mitochondrial targeting presequence that, upon removal, would result in a deduced mature protein of 347 amino acids with a molecular mass of about 39 kD(PMID:11389890). Defects in LIAS are a cause of pyruvate dehydrogenase lipoic acid synthetase deficiency (PDHLD).