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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LIG4 Monoclonal antibody

LIG4 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H6C11

Cat no : 66705-1-Ig

Synonyms

DNA ligase 4, DNA ligase IV, LIG4, ligase IV, DNA, ATP dependent

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using LIG4 Monoclonal antibody (66705-1-Ig)

    WB analysis of PC-3 using 66705-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LIG4 Monoclonal antibody (66705-1-Ig)

    WB analysis of HeLa using 66705-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using LIG4 Monoclonal antibody (66705-1-Ig)

    WB analysis of HepG2 using 66705-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using LIG4 Monoclonal antibody (66705-1-Ig)

    WB analysis of Jurkat using 66705-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Ramos cells using LIG4 Monoclonal antibody (66705-1-Ig)

    WB analysis of Ramos using 66705-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using LIG4 Monoclonal antibody (66705-1-Ig)

    WB analysis of human testis using 66705-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LIG4 Monoclonal antibody (66705-1-Ig)

    IF Staining of HepG2 using 66705-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66705-1-Ig (LIG4 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inPC-3 cells, HeLa cells, HepG2 cells, Jurkat cells, Ramos cells, human testis tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66705-1-Ig targets LIG4 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen LIG4 fusion protein Ag3385 相同性解析による交差性が予測される生物種
Full Name ligase IV, DNA, ATP-dependent
Calculated molecular weight 911 aa, 104 kDa
Observed molecular weight 100-104 kDa
GenBank accession numberBC037491
Gene symbol LIG4
Gene ID (NCBI) 3981
RRIDAB_2882057
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand brdownloadeaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.

Protocols

Product Specific Protocols
WB protocol for LIG4 antibody 66705-1-IgDownload protocol
IF protocol for LIG4 antibody 66705-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols