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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LILRB2 Polyclonal antibody

LILRB2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14154-1-AP

Synonyms

Immunoglobulin-like transcript 4, ILT-4, ILT4, ILT 4, CD85d

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using LILRB2 Polyclonal antibody (14154-1-AP)

    WB analysis of THP-1 using 14154-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 14154-1-AP (LILRB2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using LILRB2 Polyclonal antibody (14154-1-AP)

    IF Staining of THP-1 using 14154-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using LILRB2 antibody (14154-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inTHP-1 cells
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14154-1-AP targets LILRB2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LILRB2 fusion protein Ag5336 相同性解析による交差性が予測される生物種
Full Name leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2
Calculated molecular weight 598 aa, 65 kDa
Observed molecular weight60-65 kDa
GenBank accession numberBC036827
Gene symbol LILRB2
Gene ID (NCBI) 10288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LILRB2 (Leukocyte immunoglobulin-like receptor subfamily B member 2), also known as ILT4, CD85d or MIR10, is a type I transmembrane glycoprotein that contains four extracellular immunoglobulin domains, a transmembrane domain, and three cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (PMID: 33928233). LILRB2 is mainly expressed in immunocytes including monocytes, macrophages, and dendritic cells, and transduces negative signals by its association with SHP-1 phosphatase via the ITIMs (PMID: 35717259). LILRB2 can interact with various ligands such as HLA-A, HLA-B, HLA-C, HLA-G, HLA-F, CD1c, CD1d, angiopoietin-like proteins (ANGPTLs), and SEMA4A.

Protocols

Product Specific Protocols
WB protocol for LILRB2 antibody 14154-1-APDownload protocol
IF protocol for LILRB2 antibody 14154-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols