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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LIMK2 Recombinant antibody

LIMK2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230283D10

Cat no : 82977-1-RR

Synonyms

LIMK-2, LIMK 2, LIM domain kinase 2, EC:2.7.11.1, 230283D10

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LIMK2 Recombinant antibody (82977-1-RR)

    IF Staining of HepG2 using 82977-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LIMK2 antibody (82977-1-RR, Clone: 230283D10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Flow cytometry (FC) experiment of HepG2 cells using LIMK2 Recombinant antibody (82977-1-RR)

    FC experiment of HepG2 using 82977-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LIMK2 Recombinant antibody (82977-1-RR, Clone:230283D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82977-1-RR

    Affinity and Kinetic Characterization of 82977-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82977-1-RR against Human LIMK2 were performed. The affinity constant is 4.03 nM.

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82977-1-RR targets LIMK2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LIMK2 fusion protein Ag3011 相同性解析による交差性が予測される生物種
Full Name LIM domain kinase 2
Calculated molecular weight 686 aa, 78 kDa
GenBank accession numberBC013051
Gene symbol LIMK2
Gene ID (NCBI) 3985
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for LIMK2 antibody 82977-1-RRDownload protocol
FC protocol for LIMK2 antibody 82977-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols