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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LIMK2 Recombinant antibody, PBS Only (Capture)

LIMK2 Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230283H8

Cat no : 82977-2-PBS

Synonyms

LIMK-2, LIMK 2, LIM domain kinase 2, EC:2.7.11.1, 230283H8

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01226-2

    Sandwich ELISA standard curve of MP01226-2

    Sandwich ELISA standard curve of MP01226-2, Human LIMK2 Recombinant Matched Antibody Pair - PBS only. 82977-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3011. 82977-3-PBS was HRP conjugated as the detection antibody. Range: 0.39-25 ng/mL.

  • Sample test of MP01226-2

    Sample test of MP01226-2

    The mean LIMK2 concentration was determined to be 77.33 ng/mL in HepG2 cell extract based on a 1.20 mg/mL extract load and 122.10 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82977-2-PBS targets LIMK2 as part of a matched antibody pair:

MP01226-2: 82977-2-PBS capture and 82977-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LIMK2 fusion protein Ag3011 相同性解析による交差性が予測される生物種
Full Name LIM domain kinase 2
Calculated molecular weight 686 aa, 78 kDa
GenBank accession numberBC013051
Gene symbol LIMK2
Gene ID (NCBI) 3985
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.