Featured Product
KD/KO Validated

LIMPII Polyclonal antibody

Name: LIMPII Polyclonal antibody
Brand: Proteintech
SKU: 27102-1-AP

LIMPII Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 27102-1-AP

Synonyms

AMRF, CD36 antigen like 2, CD36L2, HLGP85, LGP85, LIMP II, limp2, LIMPII, Lysosome membrane protein 2, Lysosome membrane protein II, SCARB2, SR BII

Validation Data Gallery

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AllWBIHC

WB analysis using 27102-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27102-1-AP (LIMPII antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 27102-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 27102-1-AP (LIMPII Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 27102-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 27102-1-AP (LIMPII Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 27102-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27102-1-AP (LIMPII Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 27102-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27102-1-AP (LIMPII Antibody) at dilution of 1:200 (under 40x lens).

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Cat No. 27102-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, mouse liver tissue, NIH/3T3 cells, Y79 cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IHC	See 2 publications below

Product Information

27102-1-AP targets LIMPII in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LIMPII fusion protein Ag25908 相同性解析による交差性が予測される生物種
Full Name	scavenger receptor class B, member 2
Calculated molecular weight	478 aa, 54 kDa
Observed molecular weight	75-85 kDa
GenBank accession number	BC021892
Gene symbol	SCARB2
Gene ID (NCBI)	950
RRID	AB_2880756
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for LIMPII antibody 27102-1-AP	Download protocol
IHC protocol for LIMPII antibody 27102-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Virus Res Murine models of neonatal susceptibility to a clinical strain of enterovirus A71 Authors - Jing Xie View Article
human	WB	J Virol Chemokine PF4 Inhibits EV71 and CA16 Infections at the Entry Stage. Authors - Zhichao Pei View Article
mouse	WB,IHC	Viruses A Single Mutation in the VP1 Gene of Enterovirus 71 Enhances Viral Binding to Heparan Sulfate and Impairs Viral Pathogenicity in Mice. Authors - Xianliang Ke KO Validated View Article
mouse	WB, IHC	Infect Genet Evol Acarbose, as a potential drug, effectively blocked the dynamic metastasis of EV71 from the intestine to the whole body. Authors - Qingyuan Feng KD Validated View Article
mouse	WB	J Neurovirol Enterovirus A71 capsid protein VP1 increases blood-brain barrier permeability and virus receptor vimentin on the brain endothelial cells. Authors - Wenjing Wang View Article
human	WB	PLoS Pathog The apparent interferon resistance of transmitted HIV-1 is possibly a consequence of enhanced replicative fitness Authors - Elena Sugrue View Article

製品

アプリケーション

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サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

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サポートツール・試薬

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LIMPII Polyclonal antibody

LIMPII Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 27102-1-AP

WB analysis of mouse liver using 27102-1-AP

WB analysis of HepG2 using 27102-1-AP

IHC staining of human liver using 27102-1-AP

IHC staining of human liver using 27102-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Murine models of neonatal susceptibility to a clinical strain of enterovirus A71

Chemokine PF4 Inhibits EV71 and CA16 Infections at the Entry Stage.

A Single Mutation in the VP1 Gene of Enterovirus 71 Enhances Viral Binding to Heparan Sulfate and Impairs Viral Pathogenicity in Mice.

Acarbose, as a potential drug, effectively blocked the dynamic metastasis of EV71 from the intestine to the whole body.

Enterovirus A71 capsid protein VP1 increases blood-brain barrier permeability and virus receptor vimentin on the brain endothelial cells.

The apparent interferon resistance of transmitted HIV-1 is possibly a consequence of enhanced replicative fitness