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  • Featured Product
  • KD/KO Validated

LIMS1 Polyclonal antibody, PBS Only

LIMS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20772-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LIMS1 Polyclonal antibody (20772-1-AP)

    WB analysis of HeLa using 20772-1-AP (same clone as 20772-1-PBS)

    WB result of LIMS1 antibody (20772-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIMS1 transfected HeLa cells. This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Caco-2 cells using LIMS1 Polyclonal antibody (20772-1-AP)

    WB analysis of Caco-2 using 20772-1-AP (same clone as 20772-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using LIMS1 Polyclonal antibody (20772-1-AP)

    WB analysis of mouse lung using 20772-1-AP (same clone as 20772-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using LIMS1 Polyclonal antibody (20772-1-AP)

    WB analysis of human kidney using 20772-1-AP (same clone as 20772-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using LIMS1 Polyclonal antibody (20772-1-AP)

    IP experiment of K-562 using 20772-1-AP (same clone as 20772-1-PBS)

    IP result of anti-LIMS1 (IP:20772-1-AP, 4ug; Detection:20772-1-AP 1:500) with K-562 cells lysate 3600ug. This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using LIMS1 Polyclonal antibody (20772-1-AP)

    IHC staining of human oesophagus cancer using 20772-1-AP (same clone as 20772-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 20772-1-AP (LIMS1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using LIMS1 Polyclonal antibody (20772-1-AP)

    IHC staining of human oesophagus cancer using 20772-1-AP (same clone as 20772-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 20772-1-AP (LIMS1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LIMS1 Polyclonal antibody (20772-1-AP)

    IF Staining of HeLa using 20772-1-AP (same clone as 20772-1-PBS)

    Immunofluorescent analysis of Hela cells, using LIMS1 antibody 20772-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20772-1-PBS targets LIMS1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIMS1 fusion protein Ag14764 相同性解析による交差性が予測される生物種
Full Name LIM and senescent cell antigen-like domains 1
Calculated molecular weight 325 aa, 37 kDa
Observed molecular weight 35-37 kDa
GenBank accession numberBC005341
Gene symbol LIMS1
Gene ID (NCBI) 3987
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LIMS1, also known as PINCH1, is an adaptor protein that contains five LIM domains, or double zinc fingers(PMID: 7517666). PINCH-1 is a widely expressed focal adhesion protein that forms a ternary complex with integrin-linked kinase (ILK) and CH-ILKBP/actopaxin/alpha-parvin (PMID: 14551191). LIMS1 is involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques.