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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LIN28A Monoclonal antibody, PBS Only

LIN28A Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4F5G6

Cat no : 60344-1-PBS

Synonyms

LIN28, Lin-28A, Lin 28A, LIN 28, CSDD1

Validation Data Gallery

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  • Western Blot (WB) analysis of NCCIT cells using LIN28A Monoclonal antibody (60344-1-Ig)

    WB analysis of NCCIT using 60344-1-Ig (same clone as 60344-1-PBS)

    NCCIT cell were subjected to SDS PAGE followed by western blot with 60344-1-Ig (LIN28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using LIN28A Monoclonal antibody (60344-1-Ig)

    WB analysis of NCCIT using 60344-1-Ig (same clone as 60344-1-PBS)

    NCCIT cell were subjected to SDS PAGE followed by western blot with 60344-1-Ig (LIN28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using LIN28A Monoclonal antibody (60344-1-Ig)

    IHC staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60344-1-Ig (LIN28 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using LIN28A Monoclonal antibody (60344-1-Ig)

    IHC staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60344-1-Ig (LIN28 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using LIN28A Monoclonal antibody (60344-1-Ig)

    IF Staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using LIN28 antibody (60344-1-Ig, Clone: 4F5G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60344-1-PBS targets LIN28A in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LIN28A fusion protein Ag2312 相同性解析による交差性が予測される生物種
Full Name lin-28 homolog (C. elegans)
Calculated molecular weight 209 aa, 23 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC028566
Gene symbol LIN28
Gene ID (NCBI) 79727
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.