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Validation Data Gallery

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  • Western Blot (WB) analysis of NCCIT cells using LIN28A Monoclonal antibody (60344-1-Ig)

    WB analysis of NCCIT using 60344-1-Ig (same clone as 60344-1-PBS)

    NCCIT cell were subjected to SDS PAGE followed by western blot with 60344-1-Ig (LIN28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using LIN28A Monoclonal antibody (60344-1-Ig)

    WB analysis of NCCIT using 60344-1-Ig (same clone as 60344-1-PBS)

    NCCIT cell were subjected to SDS PAGE followed by western blot with 60344-1-Ig (LIN28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using LIN28A Monoclonal antibody (60344-1-Ig)

    IHC staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60344-1-Ig (LIN28 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using LIN28A Monoclonal antibody (60344-1-Ig)

    IHC staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60344-1-Ig (LIN28 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using LIN28A Monoclonal antibody (60344-1-Ig)

    IF Staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using LIN28 antibody (60344-1-Ig, Clone: 4F5G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

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LIN28A Monoclonal antibody, PBS Only

LIN28A Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 60344-1-PBS
Clone No.4F5G6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

LIN28, Lin-28A, Lin 28A, LIN 28, CSDD1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60344-1-PBS targets LIN28A in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LIN28A fusion protein Ag2312 相同性解析による交差性が予測される生物種
Full Name lin-28 homolog (C. elegans)
Calculated molecular weight 209 aa, 23 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC028566
Gene Symbol LIN28
Gene ID (NCBI) 79727
RRIDAB_2881453
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9H9Z2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.