LIN28B Polyclonal antibody
LIN28B Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, IP, ELISA, PLA
Conjugate
Unconjugated
Cat no : 24017-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, K-562 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | NCCIT cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
24017-1-AP targets LIN28B in WB, IHC, IF/ICC, IP, ELISA, PLA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LIN28B fusion protein Ag21241 相同性解析による交差性が予測される生物種 |
| Full Name | lin-28 homolog B (C. elegans) |
| Calculated molecular weight | 250 aa, 27 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | BC137526 |
| Gene symbol | LIN28B |
| Gene ID (NCBI) | 389421 |
| RRID | AB_2879403 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LIN28B, also named as CSDD2, belongs to the lin-28 family. It acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7). LIN2B is specifically expressed in embryonic muscle, neurons, and epithelia, as well as in embryonic carcinoma cells. However, it is suppressed in most adult tissues except for skeletal and cardiac muscle. The calculated molecular weight of LIN28B is 27 kDa, but a 35 kDa band has been detected in some articles (PMID: 33029224, 33378003).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LIN28B antibody 24017-1-AP | Download protocol |
| IF protocol for LIN28B antibody 24017-1-AP | Download protocol |
| IP protocol for LIN28B antibody 24017-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Immunol Lin28 regulates thymic growth and involution and correlates with MHCII expression in thymic epithelial cells | ||
iScience Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells | ||
Pharm Biol Peptides from the croceine croaker (Larimichthys crocea) swim bladder attenuate busulfan-induced oligoasthenospermia in mice. | ||
