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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using LIPC Polyclonal antibody (21133-1-AP)

    WB analysis of HepG2 using 21133-1-AP (same clone as 21133-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21133-1-AP (LIPC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LIPC Polyclonal antibody (21133-1-AP)

    WB analysis using 21133-1-AP (same clone as 21133-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21133-1-AP (LIPC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using LIPC Polyclonal antibody (21133-1-AP)

    WB analysis of mouse liver using 21133-1-AP (same clone as 21133-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21133-1-AP (LIPC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using LIPC Polyclonal antibody (21133-1-AP)

    WB analysis of mouse liver using 21133-1-AP (same clone as 21133-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21133-1-AP (LIPC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LIPC Polyclonal antibody (21133-1-AP)

    IHC staining of human lung cancer using 21133-1-AP (same clone as 21133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 21133-1-AP (LIPC Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LIPC Polyclonal antibody (21133-1-AP)

    IHC staining of human lung cancer using 21133-1-AP (same clone as 21133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 21133-1-AP (LIPC Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 21133-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LIPC Polyclonal antibody (21133-1-AP)

    IF Staining of HepG2 using 21133-1-AP (same clone as 21133-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 21133-1-AP (LIPC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21133-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

LIPC Polyclonal antibody, PBS Only

LIPC Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21133-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21133-1-PBS targets LIPC in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIPC fusion protein Ag15538 相同性解析による交差性が予測される生物種
Full Name lipase, hepatic
Calculated molecular weight 499 aa, 56 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC146659
Gene Symbol LIPC
Gene ID (NCBI) 3990
RRIDAB_11041715
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11150
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

LIPC, also named as HTGL and HL, belongs to the AB hydrolase superfamily and Lipase family. It has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. LIPC is an important enzyme in HDL metabolism. It binds heparin.