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Validation Data Gallery

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  • Western Blot (WB) analysis of MKN-45 cells using LIPF Polyclonal antibody (25889-1-AP)

    WB analysis of MKN-45 using 25889-1-AP

    MKN-45 cells were subjected to SDS PAGE followed by western blot with 25889-1-AP (LIPF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LIPF Polyclonal antibody (25889-1-AP)

    WB analysis using 25889-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25889-1-AP (LIPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using LIPF Polyclonal antibody (25889-1-AP)

    IHC staining of human stomach using 25889-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using LIPF Polyclonal antibody (25889-1-AP)

    IHC staining of human stomach using 25889-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using LIPF Polyclonal antibody (25889-1-AP)

    IHC staining of human stomach using 25889-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using LIPF Polyclonal antibody (25889-1-AP)

    IHC staining of human stomach using 25889-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using LIPF Polyclonal antibody (25889-1-AP)

    IHC staining of human liver using 25889-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using LIPF Polyclonal antibody (25889-1-AP)

    IHC staining of human liver using 25889-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using LIPF Polyclonal antibody (25889-1-AP)

    IHC staining of human stomach using 25889-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using LIPF Polyclonal antibody (25889-1-AP)

    IHC staining of human stomach using 25889-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using LIPF Polyclonal antibody (25889-1-AP)

    IHC staining of human liver using 25889-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using LIPF Polyclonal antibody (25889-1-AP)

    IHC staining of human liver using 25889-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 40x lens).

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LIPF Polyclonal antibody

LIPF Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25889-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

lipase, gastric, HLAL, HGL, GL, Gastric lipase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMKN-45 cells, mouse stomach tissue
Positive IHC detected inhuman stomach tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25889-1-AP targets LIPF in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIPF fusion protein Ag23149 相同性解析による交差性が予測される生物種
Full Name lipase, gastric
Observed molecular weight 49 kDa
GenBank accession numberBC112272
Gene Symbol LIPF
Gene ID (NCBI) 8513
RRIDAB_2880284
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07098
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

gastric lipase, is an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions.

Protocols

Product Specific Protocols
WB protocol for LIPF antibody 25889-1-APDownload protocol
IHC protocol for LIPF antibody 25889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols