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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LIPH Monoclonal antibody, PBS Only

LIPH Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F5D10

Cat no : 66303-1-PBS

Synonyms

AH, Lipase member H, lipase, member H, LIPH, LPD lipase related protein, LPDLR, mPA PLA1, mPA PLA1 alpha, MPAPLA1, Phospholipase A1 member B, PLA1B

Validation Data Gallery

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  • Western Blot (WB) analysis of SW 1990 cells using LIPH Monoclonal antibody (66303-1-Ig)

    WB analysis of SW 1990 using 66303-1-Ig (same clone as 66303-1-PBS)

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 66303-1-Ig (LIPH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66303-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of BxPC-3 cells using LIPH Monoclonal antibody (66303-1-Ig)

    WB analysis of BxPC-3 using 66303-1-Ig (same clone as 66303-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 66303-1-Ig (LIPH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66303-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using LIPH Monoclonal antibody (66303-1-Ig)

    WB analysis of COLO 320 using 66303-1-Ig (same clone as 66303-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66303-1-Ig (LIPH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66303-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using LIPH Monoclonal antibody (66303-1-Ig)

    IHC staining of human pancreas using 66303-1-Ig (same clone as 66303-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66303-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using LIPH Monoclonal antibody (66303-1-Ig)

    IHC staining of human pancreas using 66303-1-Ig (same clone as 66303-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66303-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LIPH Monoclonal antibody (66303-1-Ig)

    IHC staining of human colon cancer using 66303-1-Ig (same clone as 66303-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66303-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LIPH Monoclonal antibody (66303-1-Ig)

    IHC staining of human colon cancer using 66303-1-Ig (same clone as 66303-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66303-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using LIPH Monoclonal antibody (66303-1-Ig)

    IF Staining of BxPC-3 using 66303-1-Ig (same clone as 66303-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed BxPC-3 cells using 66303-1-Ig(LIPH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66303-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66303-1-PBS targets LIPH in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen LIPH fusion protein Ag24009 相同性解析による交差性が予測される生物種
Full Name lipase, member H
Calculated molecular weight 51 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC064941
Gene symbol LIPH
Gene ID (NCBI) 200879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LIPH(Lipase member H) is also named as LPDLR, MPAPLA1, PLA1B. It is a phosphatidic acid-selective phospholipase A1 (PLA1) that produces 2-acyl lysophosphatidic acid (LPA) and belongs to the AB hydrolase superfamily and Lipase family.LIPH has a 12-residue lid region, which likely covers a catalytic pocket, and 4 potential N-linked glycosylation sites. Defects in LIPH are the cause of hypotrichosis type 7 (HYPT7).