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Species Reactivity

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  • Featured Product
  • KD/KO Validated

LITAF Polyclonal antibody

LITAF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16797-1-AP

Synonyms

PIG7, p53-induced gene 7 protein, p53 induced gene 7 protein, LPS-induced TNF-alpha factor, LPS induced TNF alpha factor

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LITAF Polyclonal antibody (16797-1-AP)

    WB analysis of HeLa using 16797-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using LITAF Polyclonal antibody (16797-1-AP)

    WB analysis of HepG2 using 16797-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LITAF Polyclonal antibody (16797-1-AP)

    WB analysis of HeLa using 16797-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LITAF Polyclonal antibody (16797-1-AP)

    WB analysis of HeLa using 16797-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using LITAF Polyclonal antibody (16797-1-AP)

    WB analysis of HepG2 using 16797-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using LITAF Polyclonal antibody (16797-1-AP)

    WB analysis of HepG2 using 16797-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using LITAF Polyclonal antibody (16797-1-AP)

    IP experiment of HeLa using 16797-1-AP

    IP result of anti-LITAF (IP:16797-1-AP, 4ug; Detection:16797-1-AP 1:600) with HeLa cells lysate 1520ug.

  • Immunohistochemistry (IHC) staining of human spleen tissue using LITAF Polyclonal antibody (16797-1-AP)

    IHC staining of human spleen using 16797-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16797-1-AP (LITAF antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using LITAF Polyclonal antibody (16797-1-AP)

    IHC staining of human spleen using 16797-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16797-1-AP (LITAF antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LITAF Polyclonal antibody (16797-1-AP)

    IF Staining of HepG2 using 16797-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16797-1-AP (LITAF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16797-1-AP targets LITAF in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LITAF fusion protein Ag10269 相同性解析による交差性が予測される生物種
Full Name lipopolysaccharide-induced TNF factor
Calculated molecular weight 161 aa, 17 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC008309
Gene symbol LITAF
Gene ID (NCBI) 9516
RRIDAB_2135710
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lipopolysaccharide-induced TNF factor(LITAF) is a transcription factor that identified as a regulator of TNF-alpha gene expression. It may has a role in protein degradation pathways and in tumor necrosis factor alpha(TNF-alpha) gene expression. Also it may regulate the expression of the CCL2/MCP-1 chemokine through NFKB1.Defects in LITAF may be involved in extramammary Paget disease (EMPD) carcinogenesis

Protocols

Product Specific Protocols
WB protocol for LITAF antibody 16797-1-APDownload protocol
IHC protocol for LITAF antibody 16797-1-APDownload protocol
IF protocol for LITAF antibody 16797-1-APDownload protocol
IP protocol for LITAF antibody 16797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Mol Neurobiol

LITAF Enhances Radiosensitivity of Human Glioma Cells via the FoxO1 Pathway.

Authors - Changlin Huang
  • KD Validated
View Article
humanIHC

Nat Commun

Single cell transcriptomic analyses implicate an immunosuppressive tumor microenvironment in pancreatic cancer liver metastasis

Authors - Shu Zhang
View Article