Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 16797-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16797-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16797-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16797-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16797-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 16797-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16797-1-AP
IP result of anti-LITAF (IP:16797-1-AP, 4ug; Detection:16797-1-AP 1:600) with HeLa cells lysate 1520ug.
IHC staining of human spleen using 16797-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16797-1-AP (LITAF antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 16797-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16797-1-AP (LITAF antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 16797-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16797-1-AP (LITAF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

LITAF Polyclonal antibody

LITAF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16797-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PIG7, p53-induced gene 7 protein, p53 induced gene 7 protein, LPS-induced TNF-alpha factor, LPS induced TNF alpha factor

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IHC	See 1 publications below

Product Information

16797-1-AP targets LITAF in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LITAF fusion protein Ag10269 相同性解析による交差性が予測される生物種
Full Name	lipopolysaccharide-induced TNF factor
Calculated molecular weight	161 aa, 17 kDa
Observed molecular weight	24 kDa
GenBank accession number	BC008309
Gene Symbol	LITAF
Gene ID (NCBI)	9516
RRID	AB_2135710
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99732
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Lipopolysaccharide-induced TNF factor(LITAF) is a transcription factor that identified as a regulator of TNF-alpha gene expression. It may has a role in protein degradation pathways and in tumor necrosis factor alpha(TNF-alpha) gene expression. Also it may regulate the expression of the CCL2/MCP-1 chemokine through NFKB1.Defects in LITAF may be involved in extramammary Paget disease (EMPD) carcinogenesis

Protocols

Product Specific Protocols
WB protocol for LITAF antibody 16797-1-AP	Download protocol
IHC protocol for LITAF antibody 16797-1-AP	Download protocol
IF protocol for LITAF antibody 16797-1-AP	Download protocol
IP protocol for LITAF antibody 16797-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Mol Neurobiol LITAF Enhances Radiosensitivity of Human Glioma Cells via the FoxO1 Pathway. Authors - Changlin Huang KD Validated View Article
human	IHC	Nat Commun Single cell transcriptomic analyses implicate an immunosuppressive tumor microenvironment in pancreatic cancer liver metastasis Authors - Shu Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 16797-1-AP

WB analysis of HepG2 using 16797-1-AP

WB analysis of HeLa using 16797-1-AP

WB analysis of HeLa using 16797-1-AP

WB analysis of HepG2 using 16797-1-AP

WB analysis of HepG2 using 16797-1-AP

IP experiment of HeLa using 16797-1-AP

IHC staining of human spleen using 16797-1-AP

IHC staining of human spleen using 16797-1-AP

IF Staining of HepG2 using 16797-1-AP

LITAF Polyclonal antibody

LITAF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

LITAF Enhances Radiosensitivity of Human Glioma Cells via the FoxO1 Pathway.

Single cell transcriptomic analyses implicate an immunosuppressive tumor microenvironment in pancreatic cancer liver metastasis