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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LLGL2 Polyclonal antibody

LLGL2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15993-1-AP

Synonyms

LLGL scribble cell polarity complex component 2, Lethal(2) giant larvae protein homolog 2, HGL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LLGL2 Polyclonal antibody (15993-1-AP)

    WB analysis using 15993-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15993-1-AP (LLGL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LLGL2 Polyclonal antibody (15993-1-AP)

    IF Staining of HepG2 using 15993-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LLGL2 antibody (15993-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, MCF-7 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15993-1-AP targets LLGL2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LLGL2 fusion protein Ag8867 相同性解析による交差性が予測される生物種
Full Name lethal giant larvae homolog 2 (Drosophila)
Calculated molecular weight356aa,40 kDa; 1020aa,113 kDa
Observed molecular weight113-120 kDa
GenBank accession numberBC010879
Gene symbol LLGL2
Gene ID (NCBI) 3993
RRIDAB_3085478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lethal giant larvae (Lgl) is a scaffolding protein that regulates the establishment of apical-basal polarity in epithelial cells. The human homologs of Lgl are known as LLGL1 and LLGL2. LLGL1 is a tumor suppressor in multiple human cancers, including hepatocellular carcinoma, malignant melanoma, and colorectal cancer (PMID: 34178676; 15735678; 16170365). LLGL2 functions as a promoter of tumor growth and not as a tumor suppressor in ER+ breast cancer (PMID: 30996345).

Protocols

Product Specific Protocols
WB protocol for LLGL2 antibody 15993-1-APDownload protocol
IF protocol for LLGL2 antibody 15993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols