Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 13364-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13364-1-AP (ERGIC-53 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 13364-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 13364-1-AP (ERGIC-53 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13364-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13364-1-AP (ERGIC-53 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 13364-1-AP
IP result of anti-ERGIC-53 (IP:13364-1-AP, 4ug; Detection:13364-1-AP 1:400) with HepG2 cells lysate 1000 ug.
IHC staining of human stomach cancer using 13364-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13364-1-AP (ERGIC-53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 13364-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13364-1-AP (ERGIC-53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 13364-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13364-1-AP (ERGIC-53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 13364-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13364-1-AP (ERGIC-53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 13364-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using ERGIC-53 antibody (13364-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of A549 using 13364-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 13364-1-AP (ERGIC-53 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

ERGIC-53 Polyclonal antibody

ERGIC-53 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13364-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

LMAN1, Intracellular mannose-specific lectin MR60, gp58, FMFD1, F5F8D

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (25)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse brain tissue, human brain tissue, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, mouse heart tissue, mouse spleen tissue, rat heart tissue, rat spleen tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 14 publications below
IHC	See 1 publications below
IF	See 15 publications below

Product Information

13364-1-AP targets ERGIC-53 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ERGIC-53 fusion protein Ag4183 相同性解析による交差性が予測される生物種
Full Name	lectin, mannose-binding, 1
Calculated molecular weight	510 aa, 54 kDa
Observed molecular weight	54 kDa
GenBank accession number	BC032330
Gene Symbol	ERGIC-53
Gene ID (NCBI)	3998
RRID	AB_2135994
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49257
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ERGIC-53 (also known as LMAN1 or MR60) is a membrane mannose-specific lectin that selectively transports its cargo proteins from ER to ER-Golgi intermediate compartment (ERGIC) and Golgi, functioning as a cargo transport receptor for glycoproteins (PMID: 24664723; 10559958). Mutations in ERGIC-53 cause combined deficiency of coagulation factors V and VIII (PMID: 9546392).

Protocols

Product Specific Protocols
WB protocol for ERGIC-53 antibody 13364-1-AP	Download protocol
IHC protocol for ERGIC-53 antibody 13364-1-AP	Download protocol
IF protocol for ERGIC-53 antibody 13364-1-AP	Download protocol
IP protocol for ERGIC-53 antibody 13364-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. Authors - Dong Huang View Article
human	WB	Cell Metab Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans. Authors - Xiao Wang View Article
mouse	WB	Nat Cell Biol Manganese regulation of COPII condensation controls circulating lipid homeostasis Authors - Xiao Wang View Article
human	IF	Nat Commun ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling Authors - Maximilian Hirschenberger View Article
mouse	IF	Nat Commun Homeostatic regulation of STING by retrograde membrane traffic to the ER. Authors - Kojiro Mukai View Article
human	WB	Nat Commun Improved secretion of glycoproteins using an N-glycan-restricted passport sequence tag recognized by cargo receptor. Authors - Hirokazu Yagi View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13364-1-AP

WB analysis of mouse brain using 13364-1-AP

WB analysis of human brain using 13364-1-AP

IP experiment of HepG2 using 13364-1-AP

IHC staining of human stomach cancer using 13364-1-AP

IHC staining of human stomach cancer using 13364-1-AP

IHC staining of human stomach cancer using 13364-1-AP

IHC staining of human stomach cancer using 13364-1-AP

IF Staining of A549 using 13364-1-AP

IF Staining of A549 using 13364-1-AP

ERGIC-53 Polyclonal antibody

ERGIC-53 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Manganese regulation of COPII condensation controls circulating lipid homeostasis

ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling

Homeostatic regulation of STING by retrograde membrane traffic to the ER.

Improved secretion of glycoproteins using an N-glycan-restricted passport sequence tag recognized by cargo receptor.