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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LMBR1L Polyclonal antibody

LMBR1L Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12154-1-AP

Synonyms

KIAA1174, LIMR, LMBR1L, Protein LMBR1L

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using LMBR1L Polyclonal antibody (12154-1-AP)

    WB analysis of mouse testis using 12154-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using LMBR1L Polyclonal antibody (12154-1-AP)

    WB analysis of mouse liver using 12154-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using LMBR1L Polyclonal antibody (12154-1-AP)

    WB analysis of rat testis using 12154-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using LMBR1L Polyclonal antibody (12154-1-AP)

    WB analysis of HepG2 using 12154-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using LMBR1L Polyclonal antibody (12154-1-AP)

    WB analysis of A549 using 12154-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using LMBR1L Polyclonal antibody (12154-1-AP)

    IP experiment of HepG2 using 12154-1-AP

    IP result of anti-LMBR1L (IP:12154-1-AP, 4ug; Detection:12154-1-AP 1:500) with HepG2 cells lysate 3200ug.

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Tested Applications

Positive WB detected inmouse testis tissue, A549 cells, HepG2 cells, mouse liver tissue, rat testis tissue
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12154-1-AP targets LMBR1L in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LMBR1L fusion protein Ag2787 相同性解析による交差性が予測される生物種
Full Name limb region 1 homolog (mouse)-like
Calculated molecular weight 489 aa, 55 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC015015
Gene symbol LMBR1L
Gene ID (NCBI) 55716
RRIDAB_2136130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LMBR1L, also known as LIMR (lipocalin-1-interacting membrane receptor), interacts with lipocalin-1 (Lcn-1), a lipocalin member produced by a number of secretoric glands and tissues and known to bind a variety of lipophilic compounds. LMBR1L is a 489-amino acid protein with a predicted molecular mass of 55 kD and 9 putative transmembrane helices. LMBR1L has been shown to mediate cellular uptake of LMBR1L, thus it is characterized as a endocytic receptor (PMID: 11287427; 12591932). Alternatively spliced transcript variants encoding different isoforms have been described.

Protocols

Product Specific Protocols
WB protocol for LMBR1L antibody 12154-1-APDownload protocol
IP protocol for LMBR1L antibody 12154-1-APDownload protocol
FC protocol for LMBR1L antibody 12154-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols