• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LMCD1 Monoclonal antibody

LMCD1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A7B3

Cat no : 67834-1-Ig

Synonyms

Dyxin, LMCD1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of U2OS cells using LMCD1 Monoclonal antibody (67834-1-Ig)

    WB analysis of U2OS using 67834-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 67834-1-Ig (LMCD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using LMCD1 Monoclonal antibody (67834-1-Ig)

    WB analysis of human placenta using 67834-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67834-1-Ig (LMCD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using LMCD1 Monoclonal antibody (67834-1-Ig)

    IHC staining of human colon using 67834-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using LMCD1 Monoclonal antibody (67834-1-Ig)

    IHC staining of mouse skeletal muscle using 67834-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using LMCD1 Monoclonal antibody (67834-1-Ig)

    IHC staining of mouse skeletal muscle using 67834-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LMCD1 Monoclonal antibody (67834-1-Ig)

    IHC staining of human colon cancer using 67834-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LMCD1 Monoclonal antibody (67834-1-Ig)

    IHC staining of human colon cancer using 67834-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using LMCD1 Monoclonal antibody (67834-1-Ig)

    IHC staining of rat skeletal muscle using 67834-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using LMCD1 Monoclonal antibody (67834-1-Ig)

    IHC staining of rat skeletal muscle using 67834-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using LMCD1 Monoclonal antibody (67834-1-Ig)

    IHC staining of human colon using 67834-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using LMCD1 Monoclonal antibody (67834-1-Ig)

    IF Staining of MCF-7 using 67834-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using LMCD1 antibody (67834-1-Ig, Clone: 2A7B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inU2OS cells, human placenta tissue
Positive IHC detected inhuman colon tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67834-1-Ig targets LMCD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LMCD1 fusion protein Ag28331 相同性解析による交差性が予測される生物種
Full Name LIM and cysteine-rich domains 1
Calculated molecular weight 41 kDa
Observed molecular weight~40 kDa
GenBank accession numberBC000646
Gene symbol LMCD1
Gene ID (NCBI) 29995
RRIDAB_2918596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LIM and cysteine-rich domains-1 (LMCD1) is a member of the LIM protein family, which contains an N-terminal cysteine-rich region, two C-terminal LIM domains and a central PET (Prickle, Espinas, and Testin) domain. LMCD1 has been reported in cardiac tissues and lung acting as a transcriptional repressor for GATA6. The mutations of LMCD1 promote cell migration and tumor metastasis in hepatocellular carcinoma. (PMID: 31501411) It has calculated molecular weight around 41kDa.

Protocols

Product Specific Protocols
WB protocol for LMCD1 antibody 67834-1-IgDownload protocol
IHC protocol for LMCD1 antibody 67834-1-IgDownload protocol
IF protocol for LMCD1 antibody 67834-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols