Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis using 12987-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 12987-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 12987-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 12987-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 12987-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12987-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 12987-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse eye using 12987-1-AP
mouse eye tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 12987-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 12987-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 12987-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 12987-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 12987-1-AP
IP result of anti-Lamin B1 (IP:12987-1-AP, 4ug; Detection:12987-1-AP 1:20000) with HeLa cells lysate 1560 ug.
IHC staining of human liver cancer using 12987-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 12987-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 12987-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 12987-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12987-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 12987-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 12987-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 12987-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney slide using 12987-1-AP (LMNB1 Antibody) at dilution of 1:50.
IHC staining of human kidney using 12987-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney slide using 12987-1-AP (LMNB1 Antibody) at dilution of 1:50.
IF Staining of human skin cancer using 12987-1-AP
Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using 12987-1-AP (Lamin B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 12987-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 12987-1-AP (Lamin B1 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 12987-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lamin B1 antibody (12987-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of NIH/3T3 using 12987-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 12987-1-AP
Immunofluorescent analysis of HepG2 cells, using LMNB1 antibody 12987-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
FC experiment of HEK-293 using 12987-1-AP
1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human Lamin B1 (12987-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

Lamin B1 Polyclonal antibody

Lamin B1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 12987-1-AP

Publications(1203)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ChIP, ELISA

Synonyms

LMNB1, LMNB, LMN2, Lamin-B1, laminB1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1203)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, U-937 cells, mouse spleen tissue, K-562 cells, Y79 cells, mouse pancreas tissue, SH-SY5Y cells, mouse kidney tissue, mouse lung tissue, mouse eye tissue, HepG2 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse heart tissue, human kidney tissue, human liver cancer tissue, human colon tissue, mouse kidney tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human skin cancer tissue
Positive IF/ICC detected in	HepG2 cells, human skin cancer tissue, NIH/3T3 cells
Positive FC (Intra) detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 1126 publications below
IHC	See 10 publications below
IF	See 63 publications below
IP	See 1 publications below
FC	See 1 publications below
ChIP	See 3 publications below

Product Information

12987-1-AP targets Lamin B1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, canine, chicken, bovine, hamster, bombyx mori
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Lamin B1 fusion protein Ag3631 相同性解析による交差性が予測される生物種
Full Name	lamin B1
Calculated molecular weight	66 kDa
Observed molecular weight	66-70 kDa
GenBank accession number	BC012295
Gene Symbol	Lamin B1
Gene ID (NCBI)	4001
ENSEMBL Gene ID	ENSG00000113368
RRID	AB_2136290
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P20700
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.

Protocols

Product Specific Protocols
WB protocol for Lamin B1 antibody 12987-1-AP	Download protocol
IHC protocol for Lamin B1 antibody 12987-1-AP	Download protocol
IF protocol for Lamin B1 antibody 12987-1-AP	Download protocol
IP protocol for Lamin B1 antibody 12987-1-AP	Download protocol
FC protocol for Lamin B1 antibody 12987-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Nat Biotechnol Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases Authors - Matthew T N Yarnall View Article
mouse	WB	Cell Res Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies Authors - Liang Guo View Article
human	IF	Nature Chromothripsis drives the evolution of gene amplification in cancer. Authors - Ofer Shoshani View Article
	WB	Nature Stella safeguards the oocyte methylome by preventing de novo methylation mediated by DNMT1. Authors - Yingfeng Li View Article
human	WB	Mol Cancer Diversifying the anthracycline class of anti-cancer drugs identifies aclarubicin for superior survival of acute myeloid leukemia patients Authors - Xiaohang Qiao View Article
mouse	WB	Immunity 25-Hydroxycholesterol regulates lysosome AMP kinase activation and metabolic reprogramming to educate immunosuppressive macrophages Authors - Jun Xiao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12987-1-AP

WB analysis using 12987-1-AP

WB analysis of U-937 using 12987-1-AP

WB analysis of mouse spleen using 12987-1-AP

WB analysis of mouse pancreas using 12987-1-AP

WB analysis of K-562 using 12987-1-AP

WB analysis of Y79 using 12987-1-AP

WB analysis of mouse eye using 12987-1-AP

WB analysis of SH-SY5Y using 12987-1-AP

WB analysis of mouse kidney using 12987-1-AP

WB analysis using 12987-1-AP

WB analysis of mouse lung using 12987-1-AP

IP experiment of HeLa using 12987-1-AP

IHC staining of human liver cancer using 12987-1-AP

IHC staining of mouse kidney using 12987-1-AP

IHC staining of human colon using 12987-1-AP

IHC staining of human colon using 12987-1-AP

IHC staining of human colon cancer using 12987-1-AP

IHC staining of mouse heart using 12987-1-AP

IHC staining of mouse heart using 12987-1-AP

IHC staining of human kidney using 12987-1-AP

IHC staining of human kidney using 12987-1-AP

IF Staining of human skin cancer using 12987-1-AP

IF Staining of HepG2 using 12987-1-AP

IF Staining of HepG2 using 12987-1-AP

IF Staining of NIH/3T3 using 12987-1-AP

IF Staining of HepG2 using 12987-1-AP

FC experiment of HEK-293 using 12987-1-AP

Lamin B1 Polyclonal antibody

Lamin B1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases

Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies

Chromothripsis drives the evolution of gene amplification in cancer.

Stella safeguards the oocyte methylome by preventing de novo methylation mediated by DNMT1.

Diversifying the anthracycline class of anti-cancer drugs identifies aclarubicin for superior survival of acute myeloid leukemia patients

25-Hydroxycholesterol regulates lysosome AMP kinase activation and metabolic reprogramming to educate immunosuppressive macrophages