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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using LMO1 Polyclonal antibody (26932-1-AP)

    WB analysis of mouse brain using 26932-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26932-1-AP (LMO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LMO1 Polyclonal antibody (26932-1-AP)

    WB analysis using 26932-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26932-1-AP (LMO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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LMO1 Polyclonal antibody

LMO1 Polyclonal Antibody for WB, ELISA
Cat No. 26932-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Cysteine rich protein TTG 1, LIM domain only protein 1, LMO 1, LMO1, RBTN1, RHOM1, Rhombotin 1, T cell translocation protein 1, TTG1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26932-1-AP targets LMO1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LMO1 fusion protein Ag25607 相同性解析による交差性が予測される生物種
Full Name LIM domain only 1 (rhombotin 1)
Calculated molecular weight 18 kDa
Observed molecular weight18 kDa
GenBank accession numberBC069793
Gene Symbol LMO1
Gene ID (NCBI) 4004
RRIDAB_3085916
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP25800
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LOM1 (LIM Domain Only 1), as a Protein Coding gene, encodes a cysteine-rich, two LIM domain transcriptional regulator. It may be involved in gene regulation within neural lineage cells potentially by direct DNA binding or by binding to other transcription factors. A chromosomal aberration involving LMO1 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL)(Uniprot). Diseases associated with LMO1 include T-Cell Acute Lymphoblastic Leukemia and Neuroblastoma (PMID:31830377). The downstream of LMO1-regulatory cascade includes a tumor-promoting LIMS1/ILK pathway, which has a potential to be a novel therapeutic target(PMID: 29695398). The molecular weight of LMO1 is 18 kDa.

Protocols

Product Specific Protocols
WB protocol for LMO1 antibody 26932-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols