Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IF detected in | embryonic kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
18278-1-AP targets LMX1B in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LMX1B fusion protein Ag13096 相同性解析による交差性が予測される生物種 |
| Full Name | LIM homeobox transcription factor 1, beta |
| Calculated molecular weight | 372 aa, 42 kDa |
| Observed molecular weight | 42-45 kDa |
| GenBank accession number | BC112120 |
| Gene Symbol | LMX1B |
| Gene ID (NCBI) | 4010 |
| RRID | AB_11232226 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60663 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LMX1B, LIM homeodomain factor 1b, functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Lmx1b is a continuously expressed, terminal selector-type factor in 5-HT neurons. It is required for maintenance of central serotonergic neurons (PMID: 17151281, PMID: 14602809). It is expressed in brain, testis, skeletal muscle, and pancreatic islets (PMID: 35417751, PMID: 18512225).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LMX1B antibody 18278-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy A monolayer hiPSC culture system for autophagy/mitophagy studies in human dopaminergic neurons. | ||
Sci Signal Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn. | ||
Neurobiol Dis Maternal Herpesviridae infection during pregnancy alters midbrain dopaminergic signatures in adult offspring. | ||
Biomed Pharmacother LMX1B involved in the radioresistance, proliferation and migration of esophageal cancer cells.
| ||
Methods Mol Biol Imaging Autophagy in hiPSC-Derived Midbrain Dopaminergic Neuronal Cultures for Parkinson's Disease Research. | ||
J Cell Biol ATG8-dependent LMX1B-autophagy crosstalk shapes human midbrain dopaminergic neuronal resilience
|