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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using LNX1 Polyclonal antibody (13124-1-AP)

    WB analysis of mouse testis using 13124-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13124-1-AP (LNX1 antibody) at dilution of 1:70 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using LNX1 Polyclonal antibody (13124-1-AP)

    WB analysis of mouse kidney using 13124-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13124-1-AP (LNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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LNX1 Polyclonal antibody

LNX1 Polyclonal Antibody for WB, ELISA
Cat No. 13124-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ligand of numb protein X 1, LNX, LNX1, MPDZ, Numb binding protein 1, PDZRN2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse testis tissue, mouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13124-1-AP targets LNX1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LNX1 fusion protein Ag3823 相同性解析による交差性が予測される生物種
Full Name ligand of numb-protein X 1
Calculated molecular weight 728 aa, 81 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC022983
Gene Symbol LNX1
Gene ID (NCBI) 84708
RRIDAB_2136987
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TBB1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LNX1 (Ligand of Numb, protein X 1) was originally identified as a binding partner of the cell-fate determinant Numb and was reported to be involved in ubiquitylation and degradation of Numb. LNX1 contains four PDZ domains and may function as a scaffold for a multi-protein complex. LNX1 has two splicing variants, LNX1p70 and LNX1p80. LNX1p70 has the N-terminal Numb binding region and four PDZ domains, whereas LNX1p80 has an additional RING-finger domain at the N-terminus followed by a Numb-binding region and four PDZ domains. This antibody can detect both LNX1p70 and LNX1p80, around 70-80 kDa and 80-100 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for LNX1 antibody 13124-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols