Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of Jurkat cells using 15440-1-AP
WB result of LONP1 antibody (15440-1-AP, 1:6000) with si-Control and si-LONP1 transfected Jurkat cells.
WB analysis using 15440-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 15440-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 15440-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15440-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 15440-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 15440-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
WB analysis of rat lung using 15440-1-AP
rat lung tissue were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
IP experiment of mouse heart using 15440-1-AP
IP result of anti-LONP1 (IP:15440-1-AP, 3ug; Detection:15440-1-AP 1:1000) with mouse heart tissue lysate 9500ug.
IHC staining of human lung cancer using 15440-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15440-1-AP (LONP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15440-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15440-1-AP (LONP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15440-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15440-1-AP (LONP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15440-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15440-1-AP (LONP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 15440-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15440-1-AP (LONP1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 15440-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (15440-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 15440-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (15440-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HepG2 using 15440-1-AP
Immunofluorescent analysis of HepG2 cells using 15440-1-AP (LONP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

LONP1 Polyclonal antibody

LONP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15440-1-AP

Publications(95)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

PIM1, Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (95)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, human heart tissue, rat heart tissue, rat lung tissue, mouse heart tissue, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 11 publications below
WB	See 85 publications below
IHC	See 3 publications below
IF	See 10 publications below
IP	See 3 publications below
CoIP	See 1 publications below

Product Information

15440-1-AP targets LONP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, monkey, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LONP1 fusion protein Ag7196 相同性解析による交差性が予測される生物種
Full Name	lon peptidase 1, mitochondrial
Calculated molecular weight	106 kDa
Observed molecular weight	100 kDa
GenBank accession number	BC000235
Gene Symbol	LONP1
Gene ID (NCBI)	9361
RRID	AB_2137152
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P36776
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then progressively degraded(PMID:15870080). LONP1 has some isoforms with the MW of 106 kDa, 100 kDa and 86 kDa.

Protocols

Product Specific Protocols
WB protocol for LONP1 antibody 15440-1-AP	Download protocol
IHC protocol for LONP1 antibody 15440-1-AP	Download protocol
IF protocol for LONP1 antibody 15440-1-AP	Download protocol
IP protocol for LONP1 antibody 15440-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature A cytosolic surveillance mechanism activates the mitochondrial UPR Authors - F X Reymond Sutandy KD Validated View Article
human	WB	Cell Metab A High-Density Human Mitochondrial Proximity Interaction Network. Authors - Hana Antonicka View Article
monkey	WB,IF	Nat Cell Biol MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control. Authors - Tim König View Article
human	WB	Mol Cell Circadian Oscillation of Sulfiredoxin in the Mitochondria. Authors - In Sup Kil KD Validated View Article
mouse	WB	Sci Adv Fidelity of translation initiation is required for coordinated respiratory complex assembly. Authors - Danielle L Rudler View Article
mouse	WB,IHC	Sci Adv Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4. Authors - Qiqi Guo View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat cells using 15440-1-AP

WB analysis using 15440-1-AP

WB analysis using 15440-1-AP

WB analysis of human heart using 15440-1-AP

WB analysis of HeLa using 15440-1-AP

WB analysis of mouse heart using 15440-1-AP

WB analysis of rat heart using 15440-1-AP

WB analysis of rat lung using 15440-1-AP

IP experiment of mouse heart using 15440-1-AP

IHC staining of human lung cancer using 15440-1-AP

IHC staining of human lung cancer using 15440-1-AP

IHC staining of human lung cancer using 15440-1-AP

IHC staining of human lung cancer using 15440-1-AP

IF Staining of HepG2 using 15440-1-AP

IF Staining of HepG2 using 15440-1-AP

IF Staining of HepG2 using 15440-1-AP

IF Staining of HepG2 using 15440-1-AP

LONP1 Polyclonal antibody

LONP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A cytosolic surveillance mechanism activates the mitochondrial UPR

A High-Density Human Mitochondrial Proximity Interaction Network.

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.

Circadian Oscillation of Sulfiredoxin in the Mitochondria.

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.