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  • KD/KO Validated

LONP1 Polyclonal antibody

LONP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15440-1-AP

Synonyms

PIM1, Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON


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Tested Applications

Positive WB detected inHeLa cells, human heart tissue, rat heart tissue, rat lung tissue, mouse heart tissue, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15440-1-AP targets LONP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LONP1 fusion protein Ag7196 相同性解析による交差性が予測される生物種
Full Name lon peptidase 1, mitochondrial
Calculated molecular weight 106 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC000235
Gene symbol LONP1
Gene ID (NCBI) 9361
RRIDAB_2137152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then progressively degraded(PMID:15870080). LONP1 has some isoforms with the MW of 106 kDa, 100 kDa and 86 kDa.

Protocols

Product Specific Protocols
WB protocol for LONP1 antibody 15440-1-APDownload protocol
IHC protocol for LONP1 antibody 15440-1-APDownload protocol
IF protocol for LONP1 antibody 15440-1-APDownload protocol
IP protocol for LONP1 antibody 15440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A cytosolic surveillance mechanism activates the mitochondrial UPR

Authors - F X Reymond Sutandy
  • KD Validated
humanWB

Cell Metab

A High-Density Human Mitochondrial Proximity Interaction Network.

Authors - Hana Antonicka
monkeyWB,IF

Nat Cell Biol

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.

Authors - Tim König
humanWB

Mol Cell

Circadian Oscillation of Sulfiredoxin in the Mitochondria.

Authors - In Sup Kil
  • KD Validated
mouseWB

Sci Adv

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

Authors - Danielle L Rudler
mouseWB,IHC

Sci Adv

Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.

Authors - Qiqi Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

B (Verified Customer) (01-28-2023)

It is okay to detect endogenous LONP1 although there are weak non specific bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse cortex lysate
LONP1 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:15440-1-AP)