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  • Featured Product
  • KD/KO Validated

LONP1 Monoclonal antibody, PBS Only (Capture)

LONP1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C6C12

Cat no : 66043-1-PBS

Synonyms

Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LONP1 Monoclonal antibody (66043-1-Ig)

    WB analysis using 66043-1-Ig (same clone as 66043-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using LONP1 Monoclonal antibody (66043-1-Ig)

    WB analysis of Jurkat using 66043-1-Ig (same clone as 66043-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using LONP1 Monoclonal antibody (66043-1-Ig)

    WB analysis of RAW 264.7 using 66043-1-Ig (same clone as 66043-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using LONP1 Monoclonal antibody (66043-1-Ig)

    WB analysis of HSC-T6 using 66043-1-Ig (same clone as 66043-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using LONP1 Monoclonal antibody (66043-1-Ig)

    WB analysis of HEK-293 using 66043-1-Ig (same clone as 66043-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using LONP1 Monoclonal antibody (66043-1-Ig)

    WB analysis of L02 using 66043-1-Ig (same clone as 66043-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    WB analysis of human liver using 66043-1-Ig (same clone as 66043-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    WB analysis of human heart using 66043-1-Ig (same clone as 66043-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    IHC staining of human liver using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    IHC staining of human heart using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    IHC staining of human heart using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    IHC staining of human liver using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LONP1 Monoclonal antibody (66043-1-Ig)

    IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using LONP1 Monoclonal antibody (66043-1-Ig)

    IF Staining of C6 using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunofluorescent analysis of C6 cells using 66043-1-Ig (LONP1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using LONP1 Monoclonal antibody (66043-1-Ig)

    IF Staining of C6 using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using LONP1 antibody (66043-1-Ig, Clone: 1C6C12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50672-1

    Cytometric bead array standard curve of MP50672-1

    Cytometric bead array standard curve of MP50672-1, LONP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66043-1-PBS. Detection antibody: 66043-2-PBS. Standard:Ag7306. Range: 1.563-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66043-1-PBS targets LONP1 as part of a matched antibody pair:

MP50672-1: 66043-1-PBS capture and 66043-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LONP1 fusion protein Ag7306 相同性解析による交差性が予測される生物種
Full Name lon peptidase 1, mitochondrial
Calculated molecular weight 106 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC000235
Gene symbol LONP1
Gene ID (NCBI) 9361
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then processively degraded(PMID:15870080).