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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse skin tissue using Loricrin Recombinant antibody (83865-1-RR)

    IHC staining of mouse skin using 83865-1-RR (same clone as 83865-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83865-1-RR (Loricrin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83865-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Loricrin Recombinant antibody (83865-1-RR)

    IHC staining of mouse skin using 83865-1-RR (same clone as 83865-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83865-1-RR (Loricrin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83865-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Loricrin Recombinant antibody (83865-1-RR)

    IF Staining of A431 using 83865-1-RR (same clone as 83865-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using LOR antibody (83865-1-RR, Clone: 240605C8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83865-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using Loricrin Recombinant antibody (83865-1-RR)

    FC experiment of A431 using 83865-1-RR (same clone as 83865-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Loricrin Recombinant antibody (83865-1-RR, Clone:240605C8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83865-1-PBS in a different storage buffer formulation.

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Loricrin Recombinant antibody, PBS Only

Loricrin Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83865-1-PBS
Clone No.240605C8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

LOR, 240605C8, LRN

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83865-1-PBS targets Loricrin in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name loricrin
Calculated molecular weight 26 kDa
GenBank accession numberNM_000427
Gene Symbol Loricrin
Gene ID (NCBI) 4014
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP23490
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Loricrin, also named LOR and LRN, is a major component of the cornified cell envelope (CE) expressed late in epidermal differentiation in the granular layers of normal skin. This antibody is specific to Loricrin.