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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using LOX Polyclonal antibody (17958-1-AP)

    WB analysis of Jurkat using 17958-1-AP (same clone as 17958-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using LOX Polyclonal antibody (17958-1-AP)

    WB analysis of A375 using 17958-1-AP (same clone as 17958-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using LOX Polyclonal antibody (17958-1-AP)

    WB analysis of mouse skeletal muscle using 17958-1-AP (same clone as 17958-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SGC-7901 cells using LOX Polyclonal antibody (17958-1-AP)

    WB analysis of SGC-7901 using 17958-1-AP (same clone as 17958-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using LOX Polyclonal antibody (17958-1-AP)

    WB analysis of NIH/3T3 using 17958-1-AP (same clone as 17958-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MKN-45 cells using LOX Polyclonal antibody (17958-1-AP)

    WB analysis of MKN-45 using 17958-1-AP (same clone as 17958-1-PBS)

    MKN-45 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using LOX Polyclonal antibody (17958-1-AP)

    IP experiment of mouse skeletal muscle using 17958-1-AP (same clone as 17958-1-PBS)

    IP result of anti-LOX (IP:17958-1-AP, 3ug; Detection:17958-1-AP 1:500) with mouse skeletal muscle tissue lysate 2000ug. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using LOX Polyclonal antibody (17958-1-AP)

    IHC staining of human stomach cancer using 17958-1-AP (same clone as 17958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using LOX Polyclonal antibody (17958-1-AP)

    IHC staining of human stomach cancer using 17958-1-AP (same clone as 17958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using LOX Polyclonal antibody (17958-1-AP)

    IHC staining of mouse eye using 17958-1-AP (same clone as 17958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using LOX Polyclonal antibody (17958-1-AP)

    IHC staining of mouse eye using 17958-1-AP (same clone as 17958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

LOX Polyclonal antibody, PBS Only

LOX Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 17958-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Protein-lysine 6-oxidase, short form, Protein-lysine 6-oxidase, long form, Protein-lysine 6-oxidase, lysyl oxidase, EC:1.4.3.13

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17958-1-PBS targets LOX in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LOX fusion protein Ag12479 相同性解析による交差性が予測される生物種
Full Name lysyl oxidase
Calculated molecular weight 417 aa, 47 kDa
Observed molecular weight 32 kDa, 45-47 kDa
GenBank accession numberBC074872
Gene Symbol LOX
Gene ID (NCBI) 4015
RRIDAB_2234541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28300
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

LOX is a member of a multigene family (LOX, LOXL, LOXL2, LOXL3, and LOXL4). Lysyl oxidase (LOX), a copper-dependent enzyme, plays a critical role in the formation and repair of the extracellularmatrix (ECM) by catalyzing the crosslinking of elastin and collagen. LOX expression appears to be upregulated in malignancies and has recently been suggested to play a role in regulating tumor metastasis. It is synthesized as a 50 kDa proenzyme, secreted, and processed into an 32 kDa mature, active enzyme and an 18-kDa propeptide.