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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LOXL2 Polyclonal antibody

LOXL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15232-1-AP

Synonyms

WS914, WS9 14, Lysyl oxidase-related protein WS9-14, LOR2, EC:1.4.3.13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LOXL2 Polyclonal antibody (15232-1-AP)

    WB analysis using 15232-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15232-1-AP (LOXL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LOXL2 Polyclonal antibody (15232-1-AP)

    WB analysis using 15232-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15232-1-AP (LOXL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using LOXL2 Polyclonal antibody (15232-1-AP)

    IHC staining of human breast cancer using 15232-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15232-1-AP (LOXL2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using LOXL2 Polyclonal antibody (15232-1-AP)

    IHC staining of human breast cancer using 15232-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15232-1-AP (LOXL2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LOXL2 Polyclonal antibody (15232-1-AP)

    IF Staining of HepG2 using 15232-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LOXL2 antibody (15232-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LOXL2 Polyclonal antibody (15232-1-AP)

    IF Staining of HepG2 using 15232-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LOXL2 antibody (15232-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inA431 cells, MDA-MB-453s cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15232-1-AP targets LOXL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LOXL2 fusion protein Ag7083 相同性解析による交差性が予測される生物種
Full Name lysyl oxidase-like 2
Calculated molecular weight 774 aa, 87 kDa
Observed molecular weight68 kDa. 100 kDa
GenBank accession numberBC000594
Gene symbol LOXL2
Gene ID (NCBI) 4017
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LOXL2, also named as WS9-14, belongs to an amine oxidase family whose members have been implicated in crosslink formation in stromal collagens and elastin, cell motility, and tumor development and progression. LOXL2 activity might be a crucial modulator of Snail, providing an additional control mechanism of EMT and tumor progression. Increased LOXL2 expression in colon and esophageal cancer may contribute to tumor progression.

Protocols

Product Specific Protocols
WB protocol for LOXL2 antibody 15232-1-APDownload protocol
IHC protocol for LOXL2 antibody 15232-1-APDownload protocol
IF protocol for LOXL2 antibody 15232-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Clin Invest

Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer

Authors - Xiaochao Tan
View Article
humanWB

J Cancer

Dihydroartemisinin Potentiates VEGFR-TKIs Antitumorigenic Effect on Osteosarcoma by Regulating Loxl2/VEGFA Expression and Lipid Metabolism Pathway

Authors - Xiaomin Ding
View Article