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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LPAR4 Polyclonal antibody

LPAR4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22165-1-AP

Synonyms

LPA4, LPA receptor 4, LPA 4, G-protein coupled receptor 23, GPR23

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LPAR4 Polyclonal antibody (22165-1-AP)

    WB analysis using 22165-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 22165-1-AP (LPAR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LPAR4 Polyclonal antibody (22165-1-AP)

    WB analysis of mouse brain using 22165-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22165-1-AP (LPAR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using LPAR4 Polyclonal antibody (22165-1-AP)

    WB analysis of HL-60 using 22165-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 22165-1-AP (LPAR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tissue using LPAR4 Polyclonal antibody (22165-1-AP)

    IHC staining of human ovary using 22165-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 22165-1-AP (LPAR4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using LPAR4 Polyclonal antibody (22165-1-AP)

    IHC staining of human ovary using 22165-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 22165-1-AP (LPAR4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of SW 1990 cells using LPAR4 Polyclonal antibody (22165-1-AP)

    IF Staining of SW 1990 using 22165-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SW 1990 cells using LPAR4 antibody (22165-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inSKOV-3 cells, mouse brain tissue, HL-60 cells, 3T3-L1 cells
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSW 1990 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

22165-1-AP targets LPAR4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LPAR4 fusion protein Ag17817 相同性解析による交差性が予測される生物種
Full Name lysophosphatidic acid receptor 4
Calculated molecular weight 370 aa, 42 kDa
Observed molecular weight 42-50 kDa
GenBank accession numberBC069996
Gene symbol LPAR4
Gene ID (NCBI) 2846
RRIDAB_11182491
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LPAR4 antibody 22165-1-APDownload protocol
IHC protocol for LPAR4 antibody 22165-1-APDownload protocol
IF protocol for LPAR4 antibody 22165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Pancreatic cancer cells upregulate LPAR4 in response to isolation stress to promote an ECM-enriched niche and support tumour initiation

Authors - Chengsheng Wu
View Article