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KD/KO Validated

LPCAT1 Polyclonal antibody

Name: LPCAT1 Polyclonal antibody
Brand: Proteintech
SKU: 16112-1-AP

LPCAT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16112-1-AP

Synonyms

Acyltransferase like 2, AYTL2, LPC acyltransferase 1, lpcat, LPCAT 1, LPCAT1, Lyso PAF acetyltransferase, LysoPAFAT, LysoPC acyltransferase 1, PFAAP3

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 16112-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 16112-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of human lung using 16112-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 16112-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 16112-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 16112-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 16112-1-AP
IP result of anti-LPCAT1 (IP:16112-1-AP, 4ug; Detection:16112-1-AP 1:4000) with mouse brain tissue lysate 1280 ug.
IP experiment of mouse brain using 16112-1-AP
IP result of anti-LPCAT1 (IP:16112-1-AP, 3ug; Detection:16112-1-AP 1:4000) with mouse brain tissue lysate 3600ug.
IHC staining of human breast cancer using 16112-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 16112-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 16112-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 16112-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 16112-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 16112-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 16112-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 16112-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 16112-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 16112-1-AP (LPCAT1 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 16112-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, human lung tissue, mouse spleen tissue, mouse brain tissue, rat brain tissue, A549 cells, mouse lung tissue, rat lung tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human breast cancer tissue, mouse spleen tissue, mouse lung tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 8 publications below
WB	See 24 publications below
IHC	See 16 publications below
IF	See 9 publications below

Product Information

16112-1-AP targets LPCAT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	LPCAT1 fusion protein Ag9035 相同性解析による交差性が予測される生物種
Full Name	lysophosphatidylcholine acyltransferase 1
Calculated molecular weight	534 aa, 59 kDa
Observed molecular weight	59 kDa
GenBank accession number	BC020166
Gene symbol	LPCAT1
Gene ID (NCBI)	79888
RRID	AB_2135554
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LPCAT1, also named as AYTL2, PFAAP3 and LysoPAFAT, belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. It is a key enzyme for remodeling phospholipids, including phosphatidylcholine. The expression level of LPCAT1 is able to differentiate prostate cancer from noncancerous prostatic changes, and correlates to the tumor grade of prostate cancer. LPCAT1 possesses both acyltransferase and acetyltransferase activities. It mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC).

Protocols

Product Specific Protocols
WB protocol for LPCAT1 antibody 16112-1-AP	Download protocol
IHC protocol for LPCAT1 antibody 16112-1-AP	Download protocol
IF protocol for LPCAT1 antibody 16112-1-AP	Download protocol
IP protocol for LPCAT1 antibody 16112-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Cell Metab Oncogene Amplification in Growth Factor Signaling Pathways Renders Cancers Dependent on Membrane Lipid Remodeling. Authors - Junfeng Bi KD Validated View Article
human	WB	J Hepatol Lysophosphatidylcholine acyltransferase 1 altered phospholipid composition and regulated hepatoma progression. Authors - Morita Yoshifumi Y KD Validated View Article
HUMAN	WB	Curr Biol Neutral lipid stores and lipase PNPLA5 contribute to autophagosome biogenesis. Authors - Nicolas Dupont View Article
human	WB,IF	Proc Natl Acad Sci U S A Regulation of stem cell function and neuronal differentiation by HERV-K via mTOR pathway. Authors - Tongguang Wang KD Validated View Article
human	IHC	Elife Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids. Authors - Marko Z Nikolić View Article
human	WB	Front Immunol Identification of molecular subtypes based on PANoptosis-related genes and construction of a signature for predicting the prognosis and response to immunotherapy response in hepatocellular carcinoma Authors - Jinfeng Zhu KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LPCAT1 Polyclonal antibody

LPCAT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 16112-1-AP

WB analysis using 16112-1-AP

WB analysis of human lung using 16112-1-AP

WB analysis of mouse spleen using 16112-1-AP

WB analysis of mouse brain using 16112-1-AP

WB analysis of rat brain using 16112-1-AP

IP experiment of mouse brain using 16112-1-AP

IP experiment of mouse brain using 16112-1-AP

IHC staining of human breast cancer using 16112-1-AP

IHC staining of human colon cancer using 16112-1-AP

IHC staining of mouse lung using 16112-1-AP

IHC staining of human breast cancer using 16112-1-AP

IHC staining of mouse spleen using 16112-1-AP

IHC staining of mouse spleen using 16112-1-AP

IHC staining of mouse lung using 16112-1-AP

IHC staining of human colon cancer using 16112-1-AP

IF Staining of MCF-7 using 16112-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Oncogene Amplification in Growth Factor Signaling Pathways Renders Cancers Dependent on Membrane Lipid Remodeling.

Lysophosphatidylcholine acyltransferase 1 altered phospholipid composition and regulated hepatoma progression.

Neutral lipid stores and lipase PNPLA5 contribute to autophagosome biogenesis.

Regulation of stem cell function and neuronal differentiation by HERV-K via mTOR pathway.

Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids.

Identification of molecular subtypes based on PANoptosis-related genes and construction of a signature for predicting the prognosis and response to immunotherapy response in hepatocellular carcinoma