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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LPCAT1 Monoclonal antibody (66044-1-Ig)

    WB analysis using 66044-1-Ig (same clone as 66044-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using LPCAT1 Monoclonal antibody (66044-1-Ig)

    WB analysis of MCF-7 using 66044-1-Ig (same clone as 66044-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using LPCAT1 Monoclonal antibody (66044-1-Ig)

    WB analysis of ROS1728 using 66044-1-Ig (same clone as 66044-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using LPCAT1 Monoclonal antibody (66044-1-Ig)

    WB analysis of PC-12 using 66044-1-Ig (same clone as 66044-1-PBS)

    PC-12, C6, Raw264.7, NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using LPCAT1 Monoclonal antibody (66044-1-Ig)

    WB analysis of MCF-7 using 66044-1-Ig (same clone as 66044-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LPCAT1 Monoclonal antibody (66044-1-Ig)

    IHC staining of human lung cancer using 66044-1-Ig (same clone as 66044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LPCAT1 Monoclonal antibody (66044-1-Ig)

    IHC staining of human lung cancer using 66044-1-Ig (same clone as 66044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LPCAT1 Monoclonal antibody (66044-1-Ig)

    IHC staining of human liver cancer using 66044-1-Ig (same clone as 66044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LPCAT1 Monoclonal antibody (66044-1-Ig)

    IHC staining of human liver cancer using 66044-1-Ig (same clone as 66044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using LPCAT1 Monoclonal antibody (66044-1-Ig)

    IF Staining of mouse lung using 66044-1-Ig (same clone as 66044-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using LPCAT1 antibody (66044-1-Ig, Clone: 8B6E9 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), SFTPC antibody (10774-1-AP, green). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LPCAT1 Monoclonal antibody (66044-1-Ig)

    IF Staining of HeLa using 66044-1-Ig (same clone as 66044-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LPCAT1 antibody (66044-1-Ig, Clone: 8B6E9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50776-2

    Cytometric bead array standard curve of MP50776-2

    Cytometric bead array standard curve of MP50776-2, LPCAT1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66044-1-PBS. Detection antibody: 66044-3-PBS. Standard:Ag9060. Range: 0.391-25 ng/mL.

  • Cytometric bead array standard curve of MP50776-3

    Cytometric bead array standard curve of MP50776-3

    Cytometric bead array standard curve of MP50776-3, LPCAT1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66044-1-PBS. Detection antibody: 66044-2-PBS. Standard:Ag9060. Range: 0.391-100 ng/mL.

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  • Featured Product
  • KD/KO Validated

LPCAT1 Monoclonal antibody, PBS Only (Capture)

LPCAT1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 66044-1-PBS
Clone No.8B6E9

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

Acetyl-CoA:lyso-PAF acetyltransferase, 8B6E9, 1-alkylglycerophosphocholine O-acetyltransferase, 1-alkenylglycerophosphocholine O-acyltransferase, 1-acylglycerophosphocholine O-acyltransferase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66044-1-PBS targets LPCAT1 as part of a matched antibody pair:

MP50776-2: 66044-1-PBS capture and 66044-3-PBS detection (validated in Cytometric bead array)

MP50776-3: 66044-1-PBS capture and 66044-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen LPCAT1 fusion protein Ag9060 相同性解析による交差性が予測される生物種
Full Name lysophosphatidylcholine acyltransferase 1
Calculated molecular weight 534 aa, 59 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC020166
Gene Symbol LPCAT1
Gene ID (NCBI) 79888
RRIDAB_11045658
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NF37
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

LPCAT1, also named as AYTL2, PFAAP3 and LysoPAFAT, belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. It is a key enzyme for remodeling phospholipids, including phosphatidylcholine. The expression level of LPCAT1 is able to differentiate prostate cancer from noncancerous prostatic changes, and correlates to the tumor grade of prostate cancer. LPCAT1 possesses both acyltransferase and acetyltransferase activities. It mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC).