Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, LPS treated HEK-293, HeLa cells, Jurkat cells, RAW 264.7 cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
15082-1-AP targets LPCAT2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LPCAT2 fusion protein Ag7156 相同性解析による交差性が予測される生物種 |
| Full Name | lysophosphatidylcholine acyltransferase 2 |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 53 kDa |
| GenBank accession number | BC002472 |
| Gene Symbol | LPCAT2 |
| Gene ID (NCBI) | 54947 |
| RRID | AB_2135557 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7L5N7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LPCAT2 antibody 15082-1-AP | Download protocol |
| IF protocol for LPCAT2 antibody 15082-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Antioxidants (Basel) Mitochondrial Reactive Oxygen Species Formation Determines ACSL4/LPCAT2-Mediated Ferroptosis |