LPCAT2 Polyclonal antibody
LPCAT2 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 15082-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, LPS treated HEK-293, HeLa cells, Jurkat cells, RAW 264.7 cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
15082-1-AP targets LPCAT2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LPCAT2 fusion protein Ag7156 相同性解析による交差性が予測される生物種 |
| Full Name | lysophosphatidylcholine acyltransferase 2 |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 53 kDa |
| GenBank accession number | BC002472 |
| Gene symbol | LPCAT2 |
| Gene ID (NCBI) | 54947 |
| RRID | AB_2135557 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LPCAT2 antibody 15082-1-AP | Download protocol |
| IF protocol for LPCAT2 antibody 15082-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Antioxidants (Basel) Mitochondrial Reactive Oxygen Species Formation Determines ACSL4/LPCAT2-Mediated Ferroptosis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Melanie (Verified Customer) (04-22-2022) | LPCAT2 antibody was diluted 1:1500 in 5% milk in TBST and applied to protein samples from Hek293T cells. The bands were very clear. The antibody could have been diluted even more.
|
FH Christian (Verified Customer) (12-18-2018) | 10 μg protein per lane run on 10% Tris-Glycine gel. Primary incubation 1:400 overnight @ 4°C. Secondary antibody and biotinylated marker were supplied from a different vendor and used and used at optimized dilutions.LPCAT2 siRNA was used to check antibody specificity.The antibody performed excellently, will try at a dilution of 1:800 next time.
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