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Validation Data Gallery

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  • Western Blot (WB) analysis of pig stomach tissue using LPCAT3 Monoclonal antibody (67882-1-Ig)

    WB analysis of pig stomach using 67882-1-Ig (same clone as 67882-1-PBS)

    pig stomach tissue were subjected to SDS PAGE followed by western blot with 67882-1-Ig (LPCAT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67882-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using LPCAT3 Monoclonal antibody (67882-1-Ig)

    WB analysis of HepG2 using 67882-1-Ig (same clone as 67882-1-PBS)

    WB result of LPCAT3 antibody (67882-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LPCAT3 transfected HepG2 cells. This data was developed using the same antibody clone with 67882-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using LPCAT3 Monoclonal antibody (67882-1-Ig)

    IHC staining of human stomach using 67882-1-Ig (same clone as 67882-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 67882-1-Ig (LPCAT3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67882-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using LPCAT3 Monoclonal antibody (67882-1-Ig)

    IHC staining of human stomach using 67882-1-Ig (same clone as 67882-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 67882-1-Ig (LPCAT3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67882-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using LPCAT3 Monoclonal antibody (67882-1-Ig)

    IHC staining of human small intestine using 67882-1-Ig (same clone as 67882-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67882-1-Ig (LPCAT3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67882-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using LPCAT3 Monoclonal antibody (67882-1-Ig)

    IHC staining of human small intestine using 67882-1-Ig (same clone as 67882-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67882-1-Ig (LPCAT3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67882-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

LPCAT3 Monoclonal antibody, PBS Only

LPCAT3 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67882-1-PBS
Clone No.1C1G3

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

EC:2.3.1.-, C3F, 1C1G3, 1-acylglycerophosphoserine O-acyltransferase, 1-acylglycerophosphoethanolamine O-acyltransferase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67882-1-PBS targets LPCAT3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LPCAT3 fusion protein Ag6472 相同性解析による交差性が予測される生物種
Full Name lysophosphatidylcholine acyltransferase 3
Calculated molecular weight 56 kDa
Observed molecular weight56 kDa
GenBank accession numberBC065194
Gene Symbol LPCAT3
Gene ID (NCBI) 10162
RRIDAB_2918639
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6P1A2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Lysophosphatidylcholine acyltransferases (LPCATs) are among the lysophopholipid acyltransferases (LPLATs) that play an important role in lipid metabolism and homeostasis by regulating the abundance of different phosphatidylcholine (PC) species. Lysophosphatidylcholine acyltransferase 3 (LPCAT3) is a member of the LPCAT family that primarily regulates the levels of arachidonic PC species. LPCAT3 is abundantly expressed in the testes, kidneys, and metabolic tissues, including the liver, intestine, and adipose tissues. LPCAT3 is involved in the occurrence and development of atherosclerosis, intestinal tumors, and nonalcoholic steatohepatitis (NASH)(PMID: 35711841).