LPCAT4 Polyclonal antibody

LPCAT4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17905-1-AP

Synonyms

1-AGP acyltransferase 7, 1-acylglycerophosphoserine O-acyltransferase, 1-acylglycerophosphocholine O-acyltransferase, 1-acylglycerol-3-phosphate O-acyltransferase 7, 1 AGPAT 7


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Tested Applications

Positive WB detected inHT-29 cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

17905-1-AP targets LPCAT4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LPCAT4 fusion protein Ag12281 相同性解析による交差性が予測される生物種
Full Name lysophosphatidylcholine acyltransferase 4
Calculated molecular weight 524 aa, 57 kDa
Observed molecular weight 50-57 kDa
GenBank accession numberBC092463
Gene symbol LPCAT4
Gene ID (NCBI) 254531
RRIDAB_2140478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LPCAT4, also known as lysophosphatidylcholine acyltransferase 4, is an enzyme that plays a crucial role in lipid metabolism and membrane remodeling It is a member of the 1-acylglycerol-3-phosphate O-acyltransferase (EC 2.3.1.51) family and is involved in the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA), a precursor in the biosynthesis of all glycerolipids. Both LPA and PA are involved in signal transduction. The protein is predicted to be located in the membrane and is classified as a metabolic protein and enzyme. It is involved in biological processes such as lipid biosynthesis, lipid metabolism, phospholipid biosynthesis, and phospholipid metabolism. .

Protocols

Product Specific Protocols
WB protocol for LPCAT4 antibody 17905-1-APDownload protocol
IHC protocol for LPCAT4 antibody 17905-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Hepatol

Lysophosphatidylcholine acyltransferase 1 altered phospholipid composition and regulated hepatoma progression.

Authors - Morita Yoshifumi Y
mouseWB

Neurochem Res

iPLA2β Knockout Mouse, a Genetic Model for Progressive Human Motor Disorders, Develops Age-Related Neuropathology.

Authors - Helene Blanchard
humanWB

FASEB J

Deep multilayer brain omics identifies the potential involvement of menopause molecular networks in Gliomas' disease progression

Authors - Chunhua Liu
humanWB

Front Biosci (Landmark Ed)

Upregulation of LPGAT1 Enhances Lung Adenocarcinoma Proliferation

Authors - Huiyuan Gong
mouseWB

FASEB J

Overexpression of lysophospholipid acyltransferase, LPLAT10/LPCAT4/LPEAT2, in the mouse liver increases glucose-stimulated insulin secretion

Authors - Kahori Shimizu