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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LPHN3 Polyclonal antibody

LPHN3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 20045-1-AP

Synonyms

CIRL 3, CIRL3, KIAA0768, latrophilin 3, LEC3, Lectomedin 3, LPHN3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using LPHN3 Polyclonal antibody (20045-1-AP)

    WB analysis of mouse brain using 20045-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using LPHN3 Polyclonal antibody (20045-1-AP)

    WB analysis of mouse colon using 20045-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using LPHN3 Polyclonal antibody (20045-1-AP)

    WB analysis of HepG2 using 20045-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using LPHN3 Polyclonal antibody (20045-1-AP)

    WB analysis of COLO 320 using 20045-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using LPHN3 Polyclonal antibody (20045-1-AP)

    WB analysis of rat brain using 20045-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using LPHN3 Polyclonal antibody (20045-1-AP)

    WB analysis of human liver using 20045-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using LPHN3 Polyclonal antibody (20045-1-AP)

    IP experiment of mouse brain using 20045-1-AP

    IP result of anti-LPHN3 (IP:20045-1-AP, 4ug; Detection:20045-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells, mouse colon tissue, COLO 320 cells, rat brain tissue, human liver tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

20045-1-AP targets LPHN3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name latrophilin 3
Calculated molecular weight 162 kDa
Observed molecular weight 139 kDa
GenBank accession numberNM_015236
Gene symbol LPHN3
Gene ID (NCBI) 23284
RRIDAB_10665552
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LPHN3, also named as KIAA0768 and LEC3, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. LPHN3 forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85). This antibody recognizes all the isoforms of LPHN3 and has no cross reaction with LPHN1 and LPHN2.

Protocols

Product Specific Protocols
WB protocol for LPHN3 antibody 20045-1-APDownload protocol
IP protocol for LPHN3 antibody 20045-1-APDownload protocol
FC protocol for LPHN3 antibody 20045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

World J Biol Psychiatry

Expression of the adult ADHD-associated gene ADGRL3 is dysregulated by risk variants and environmental risk factors.

Authors - Rhiannon V McNeill
View Article
humanWB

Nat Microbiol

Brain exposure to SARS-CoV-2 virions perturbs synaptic homeostasis

Authors - Emma Partiot
View Article
human

Sci Adv

The FLRT3-UNC5B checkpoint pathway inhibits T cell-based cancer immunotherapies

Authors - Kushal Prajapati
View Article