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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LPL Polyclonal antibody, PBS Only

LPL Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28602-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LPL Polyclonal antibody (28602-1-AP)

    WB analysis using 28602-1-AP (same clone as 28602-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 28602-1-AP (LPL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28602-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using LPL Polyclonal antibody (28602-1-AP)

    IHC staining of mouse brown adipose using 28602-1-AP (same clone as 28602-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 28602-1-AP (LPL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28602-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28602-1-PBS targets LPL in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein 相同性解析による交差性が予測される生物種
Full Name lipoprotein lipase
Calculated molecular weight 475 aa, 53 kDa
Observed molecular weight55 kDa
GenBank accession numberBC011353
Gene symbol LPL
Gene ID (NCBI) 4023
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Lipoprotein lipase (LPL) is a key enzyme in lipid and lipoprotein metabolism. The canonical role of LPL involves the hydrolysis of triglyceride-rich lipoproteins for the provision of FFAs to metabolic tissues. LPL is highly expressed in adipocytes and myocytes as well as macrophages. Its protein level and activity are tightly regulated by multiple mechanisms in response to the metabolic state and energy demands of the cell . (PMID: 33277156, PMID: 33172164)