Validation Data Gallery
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Tested Applications
| Positive WB detected in | human saliva tissue, K-562 cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
10376-1-AP targets LPO in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | peptide 相同性解析による交差性が予測される生物種 |
| Full Name | lactoperoxidase |
| Calculated molecular weight | 80 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | BC107167 |
| Gene Symbol | LPO |
| Gene ID (NCBI) | 4025 |
| RRID | AB_2877734 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22079 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LPO antibody 10376-1-AP | Download protocol |
| IHC protocol for LPO antibody 10376-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS One Biochemical properties of thyroid peroxidase (TPO) expressed in human breast and mammary-derived cell lines. | ||
PLoS One Thyroid peroxidase (TPO) expressed in thyroid and breast tissues shows similar antigenic properties. | ||
Front Cell Dev Biol Identification and verification of a prognostic signature based on a miRNA-mRNA interaction pattern in colon adenocarcinoma | ||
FASEB J Melatonin suppresses atherosclerosis by ferroptosis inhibition via activating NRF2 pathway | ||
Free Radic Res Metformin suppresses metabolic dysfunction-associated fatty liver disease by ferroptosis and apoptosis via activation of oxidative stress |