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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LPP Polyclonal antibody

LPP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25045-1-AP

Synonyms

Lipoma preferred partner, LPP

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using LPP Polyclonal antibody (25045-1-AP)

    WB analysis using 25045-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LPP Polyclonal antibody (25045-1-AP)

    WB analysis using 25045-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LPP Polyclonal antibody (25045-1-AP)

    WB analysis using 25045-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using LPP Polyclonal antibody (25045-1-AP)

    WB analysis of MCF-7 using 25045-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25045-1-AP (LPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using LPP Polyclonal antibody (25045-1-AP)

    IP experiment of HeLa using 25045-1-AP

    IP result of anti-LPP (IP:25045-1-AP, 4ug; Detection:25045-1-AP 1:1000) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using LPP Polyclonal antibody (25045-1-AP)

    IHC staining of human small intestine using 25045-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using LPP Polyclonal antibody (25045-1-AP)

    IHC staining of human small intestine using 25045-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LPP Polyclonal antibody (25045-1-AP)

    IHC staining of human kidney using 25045-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LPP Polyclonal antibody (25045-1-AP)

    IHC staining of human kidney using 25045-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25045-1-AP (LPP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LPP Polyclonal antibody (25045-1-AP)

    IF Staining of HeLa using 25045-1-AP

    Immunofluorescent analysis of HeLa cells using 25045-1-AP (LPP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, NIH/3T3 cells, mouse colon, mouse heart, rat heart
Positive IP detected inHeLa cells
Positive IHC detected inhuman small intestine tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25045-1-AP targets LPP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LPP fusion protein Ag19205 相同性解析による交差性が予測される生物種
Full Name LIM domain containing preferred translocation partner in lipoma
Calculated molecular weight 612 aa, 66 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC130584
Gene symbol LPP
Gene ID (NCBI) 4026
RRIDAB_2879869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LPP antibody 25045-1-APDownload protocol
IHC protocol for LPP antibody 25045-1-APDownload protocol
IF protocol for LPP antibody 25045-1-APDownload protocol
IP protocol for LPP antibody 25045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Cell Death Dis

UBE2S interacting with TRIM21 mediates the K11-linked ubiquitination of LPP to promote the lymphatic metastasis of bladder cancer

Authors - Kanghua Xiao
View Article