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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LPXN Polyclonal antibody

LPXN Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31231-1-AP

Synonyms

LDLP, LDPL, leupaxin, LPXN

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LPXN Polyclonal antibody (31231-1-AP)

    WB analysis using 31231-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31231-1-AP (LPXN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inMDA-MB-231 cells, PC-3 cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31231-1-AP targets LPXN in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LPXN fusion protein Ag34956 相同性解析による交差性が予測される生物種
Full Name leupaxin
Calculated molecular weight 43 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC019035
Gene symbol LPXN
Gene ID (NCBI) 9404
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LPXN, also known as Leupaxin, LDPL, belongs to the paxillin family. LPXN Contributes to the regulation of cell adhesion, spreading, and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events (PMID: 20543562). LPXN has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of the focal adhesion kinase family (PMID: 9565592). Studies in breast cancer implicate LPXN is involved in the regulation of ER action as a co-factor (PMID: 25955236). LPXN is expressed in prostate cancer cells and its expression intensity is directly linked to PCa progression (PMID: 18451096).

Protocols

Product Specific Protocols
WB protocol for LPXN antibody 31231-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols