LRAT Polyclonal antibody

LRAT Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12815-1-AP

Synonyms

LRAT


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Tested Applications

Positive WB detected inmouse testis tissue, Y79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

12815-1-AP targets LRAT in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRAT fusion protein Ag3556 相同性解析による交差性が予測される生物種
Full Name lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)
Calculated molecular weight 230 aa, 26 kDa
Observed molecular weight 26 kDa, 50-54 kDa
GenBank accession numberBC031053
Gene symbol LRAT
Gene ID (NCBI) 9227
RRIDAB_2297180
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LRAT belongs to the H-rev107 family. LRAT transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, LRAT monomers (26kDa) interact in membranes and form functional homodimers (50-54 kDa) through protein-protein interactions and disulfide bond formation.Defects in LRAT are a cause of severe early-onset retinal dystrophy (RD). This antibody was generated against the internal residues of human LRAT. It is predicted to detect endogenous LRAT.

Protocols

Product Specific Protocols
WB protocol for LRAT antibody 12815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

iScience

Control by the brain of vitamin A homeostasis

Authors - Peter I Imoesi
ratWB

Am J Pathol

Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy.

Authors - Volha V Malechka
mouseWB

J Food Biochem

Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice.

Authors - Wei Li
human,mouseWB

iScience

Alphaherpesvirus manipulates retinoic acid metabolism for optimal replication

Authors - Shengli Ming
human,ratWB

Exp Eye Res

Mechanisms of retinal photoreceptor loss in spontaneously hypertensive rats

Authors - Minsup Lee

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Peter (Verified Customer) (12-03-2018)

The Proteintech LRAT primary antibody works so well and the antibody is prepared using BSA it can be use repeatedly when stored at -20. The strength of the antibody remains the same over a period of 6 months or more.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Rat Liver Tissue
LRAT Antibody Western Blot, validation (1:500 dilution) in Rat Liver Tissue (Cat no:12815-1-AP)