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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LRIG1 Polyclonal antibody

LRIG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22383-1-AP

Synonyms

LIG 1, LIG1, LRIG1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using LRIG1 Polyclonal antibody (22383-1-AP)

    WB analysis of mouse liver using 22383-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 22383-1-AP (LRIG1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse skin tissue using LRIG1 Polyclonal antibody (22383-1-AP)

    WB analysis of mouse skin using 22383-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 22383-1-AP (LRIG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using LRIG1 Polyclonal antibody (22383-1-AP)

    IHC staining of mouse kidney using 22383-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22383-1-AP (LRIG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse liver tissue, mouse skin tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22383-1-AP is LRIG1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRIG1 fusion protein Ag17880 相同性解析による交差性が予測される生物種
Full Name leucine-rich repeats and immunoglobulin-like domains 1
Calculated molecular weight 1093 aa, 119 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC071561
Gene symbol LRIG1
Gene ID (NCBI) 26018
RRIDAB_2879095
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LRIG1 acts as a feedback negative regulator of signaling by receptor tyrosine kinases, through a mechanism that involves enhancement of receptor ubiquitination and accelerated intracellular degradation.

Protocols

Product Specific Protocols
WB protocol for LRIG1 antibody 22383-1-APDownload protocol
IHC protocol for LRIG1 antibody 22383-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols