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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LRMP Polyclonal antibody

LRMP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19498-1-AP

Synonyms

Protein Jaw1, Processed inositol 1,4,5-triphosphate receptor associated 2, Lymphoid-restricted membrane protein, JAW1, IRAG2

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using LRMP Polyclonal antibody (19498-1-AP)

    WB analysis of Raji using 19498-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 19498-1-AP (LRMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using LRMP Polyclonal antibody (19498-1-AP)

    WB analysis of Jurkat using 19498-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19498-1-AP (LRMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human spleen tissue using LRMP Polyclonal antibody (19498-1-AP)

    IHC staining of human spleen using 19498-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 19498-1-AP (LRMP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using LRMP Polyclonal antibody (19498-1-AP)

    IHC staining of human spleen using 19498-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 19498-1-AP (LRMP antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using LRMP Polyclonal antibody (19498-1-AP)

    IF Staining of Jurkat using 19498-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using LRMP antibody (19498-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Flow cytometry (FC) experiment of Jurkat cells using LRMP Polyclonal antibody (19498-1-AP)

    FC experiment of Jurkat using 19498-1-AP

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug LRMP Polyclonal antibody (19498-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inRaji cells, Jurkat cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19498-1-AP targets LRMP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name lymphoid-restricted membrane protein
Calculated molecular weight 62 kDa
Observed molecular weight 70 kDa
GenBank accession numberNM_006152
Gene symbol LRMP
Gene ID (NCBI) 4033
RRIDAB_10640430
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LRMP, also named as JAW1, is a lymphoid restricted integral membrane protein that is localised to the cytoplasmic face of the endoplasmic reticulum.

Protocols

Product Specific Protocols
WB protocol for LRMP antibody 19498-1-APDownload protocol
IHC protocol for LRMP antibody 19498-1-APDownload protocol
IF protocol for LRMP antibody 19498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Sara (Verified Customer) (10-06-2024)

We tried flow cytometry (FC) with both human and mouse cells and it did now work for either. However, to be fair, the data sheet does not report FC as one of the tested applications and it also says that it does not work in mouse, so it is expected. If Proteintech could develop an anti-mouse LRMP that would be amazing, we haven't found any that works.

  • Applications: Flow Cytometry
  • Cell Tissue Type: Human and mouse